AIM:To evaluate the efficacy of tubeimoside(Tu)on herpes simplex keratitis(HSK)of rabbits.METHODS:HSK models were established in 24 rabbits,which were randomly divided into 4 groups:normal saline(NS),acyclovir(ACV),Tu and Tu combined with recombinant bovine basic fibroblast growth factor(bFGF).All eyes were examined and corneal samples’ lesions dynamic changes were selectively observed by slit-lamp microscope,scanning and transmission electron microscope.RESULTS:Among 4 groups of NS,ACV,Tu and Tu +bFGF,corneal epithelial involvement scores(CEIS)showed significant difference(P<0.01);compared ACV with Tu or Tu +bFGF respectively,CEIS had significant difference(P<0.05),as well as that between Tu and Tu +bFGF(P<0.05).CONCLUSION:There is curative effect on HSK with Tu(0.4g/L),and the therapeutic effect can be enhanced when combined with bFGF except for slight stimulatory.