AIM:To summarize and discuss the treatment experience in persistent corneal epithelium defect after vitrectomy.METHODS: The repairment of cornea by tri- or quad-kinds treatments for 10 cases 11 eyes with the persistent corneal epithelial defect after vitrectomy were evaluated. Original postoperative anti-inflammatory eyedrop was halted, instead of dropping serum eyedrop to promote corneal repair. Moderate hormone was applied by retrobulbar injection and temporary eyelid saturation. The ratio of persistent corneal epithelium defect after vitrectomy was analyzed.RESULTS: The rate of persistent corneal epithelium defect after vitrectomy was 2.2% (11/509), the rate of diabetic retinopathy case was 4.8% (8/165). The curative rate was 100%. The rate of corneal nebula was 45.5% (5/11). CONCLUSION: The reason for persistent corneal epithelium defect is complex. It’s worth paying attention to diabetes cases. To terminate drug toxicity, strengthen the nutrition of cornea and suture the eyelid temporarily are the effective treatments.