AIM:To evaluate the clinical efficacy and application value of Cyclosporin A (CsA) and 5-Fluorouracil (5-FU) in glaucoma filtration surgery through comparing the results of CsA and 5-FU in the surgery.METHODS:Sixty cases(90 eyes)with intractable glaucomatous patients were randomly studied by treatment with CsA or 5-FU following trabeculectomy,each group consisting of 30 cases(45 eyes).20g/L CsA and 25mg/mL 5-FU were used for treatment of each group respectively for 4minutes.The follow-up was ranged 15 months.RESULTS:The 15th month life-table success rate of functional bleb formation was (81.25±10.13)% in CsA group and (61.75±8.12)% in 5-FU group,the difference being statistically significant(P<0.05).The 15th month life-table complete and qualified success rates were (81.25±10.13)% and (92.62±3.38)% in CsA group,(61.75±8.12)% and (79.37±6.12)% in 5-FU group,the difference being statistically significant(P<0.05).Toxic and side effcts induced by CsA were more rare than 5-FU,No severe complications were found.CONCLUSION:CsA is more safe than 5-FU in glaucoma filtration surgery.