AIM:To create an effective operation model in glaucoma filtering operation in rabbit. METHODS:Trabeculectomy were performed on 36 eyes of 18 rabbits. The 18 eyes were treated with the amniotic transplantation under sclerotic flap, the other eyes were treated with the double amniotic transplantation between sclerotic and conjunctional flap. The postoperative examinations including the intraocular pressure (IOP), filtering bleb and complications were performed. RESULTS:Filtering bleb was scarcely enough to keep effective and permanent. In double amniotic membrane transplantation group, the filtering bleb was kept over 14 and 30 days partly, showing a longer period in comparison with amniotic membrane groups. The IOP remained steady at a level of less than baseline. CONCLUSION:The double amniotic membrane transplantation can improve success rate of model.