AIM:To investigate the expression pattern and correlation of insulin-like growth factor-1 (IGF-1)and its binding protein-3(IGFBP-3) in the ischemia-reperfusion in rats. METHODS:The models of retinal ischemia-reperfusion in rats were made.In order to analyze the correlation and expression between IGF-1 and IGFBP-3 in retinal ischemia-reperfusion model,immunohistochemical method was used. RESULTS:In the normal retina,IGF-1 and IGFBP-3 were slightly expressed,while in retinal ischemia-reperfusion group,the expression of IGF-1 ascended obviously at 6 hours,reached peak at 72 hours; the expression of IGFBP-3 ascended obviously at 12 hours,reached peak at 72 hours，and the two had a collaborative relationship. CONCLUSION: IGF-1 and IGFBP-3 expression are closely related to retinal ischemia-reperfusion injury,and have a positive correlation.