AIM:To probe into the effect of amniotic membrane implantation and mitomycinc in non-penetrating trabecular surgery (NPTS) for the treatment of primary open angle glaucoma(POAG). METHODS:Forty-two eyes of 30 POAG patients were treated with amniotic membrane implantation and mitomycinc in NPTS. RESULTS:Intraocular pressure: (35.76±6.34)mmHg before the operation, (13.82±4.22) mmHg after the operation. Visual acuity：12 eyes higher than before the operation, 26 eyes remained the same ,4 eyes worse than before. Visual field:34 eyes expanded relatively, 8 eyes remained the same. Follicle：flat bleb in 38 eyes, cyst bleb in 3 eyes, scar organized bleb in 1 eye. Anterior chamber: No shallow anterior chamber observed in all cases. CONCLUSION:Amniotic membrane implantation and mitomycin in NPTS for the treatment of POAG seems a simple, safe and effective procedure of anti-glaucoma operation.