AIM:To evaluate the clinical efficacy of protein-free calf blood extract for conjunctiva-leaking after trabeculectomy in human eyes. METHODS:A retrospective study with control designa-tion was carried out from January 2007 to December 2010.The first group was conjunctiva-leaking eyes without protein-free calf blood extract.The second group of conjunctiva-leaking eyes was treated by 20% protein-free calf blood extract eye drops.The leaking of conjunctiva was recorded on day 3,7,14. RESULTS:There was significant difference between the two groups in conjunctival healing time (P<0.05). CONCLUSION:Protein-free calf blood extract eye drops has confirmed efficacy in treating conjunctiva-leaking after trabeculectomy.