AIM:To compare the difference of intraocular pressure of non-contact tonometry and Schiotz tonometer in different corneal thickness. METHODS:Totally 314 cases (627 eyes) were recruited. Corneal thickness was measured by corneal pachymeter and intraocular pressure was measured by non-contact tonometry and Schiotz tonometer. Eyes were divided into 3 groups based on corneal thickness: thin group: corneal thickness＜530μm, normal group: 530μm＜corneal thickness ≤570μm and thick group: corneal thickness＞570μm. The statistical significance of differences was evaluated by paired t-test and the agreement was assessed by Bland-Altman analysis. RESULTS:The eyes of thin, normal and thick group were: 170, 301 and 156 eyes, respectively.The intraocular pressure of non-contact tonometry and Schiotz tonometer in 3 groups were 12.82±2.67 mmHg，12.84±2.37mmHg；1367±2.66mmHg，13.58±2.41mmHg；15.45±2.91mmHg，14.76±2.39mmHg, respectively. There was significant difference in thick group and no significant difference in other groups. CONCLUSION: It is suggested that either non-contact tonometry or Schiotz tonometer could measure intraocular pressure in eyes with corneal thickness≤570μm, and consult the intraocular pressure of the two measurements in eyes with corneal thickness ＞570μm.