AIM:To observe the short-term changes of pupil and intraocular pressure（IOP） after compound anisodine injection. METHODS:In 74 cases (148 eyes), the pupil diameter and IOP were measured by classical pupil diameter ruler and non-contact tonometer immediately before and 15, 30, 60, 120 minutes after compound anisodine injection. Statistical analysis was performed using the SPSS 18.0 program and paired t-test was used in our study. RESULTS：IOP was decreased after compound anisodine injection at least in a short time(within 120 minutes),and the decrease is statistically significant(P＜0.01).The pupil was bigger at 15^th minute after compound anisodine injection than before the therapy(P＜0.01),which is last until 60^th minute. Then the pupil became smaller. CONCLUSION:Compound anisodine superficial temporal subcutaneous injection has little effect on IOP fluctuation and IOP of most patients was decreased. Mydriasis effect of the drug is minor and short-acting, which can not induce ocular hypertension. "