AIM: To study the inhibition of Tripterygium Polyglucoside on chemical burn induced model of corneal neovascularization (CNV). METHODS: A model of CNV was created by 750mL/L silver nitrate solution burn in 20 healthy rats which were subsequently randomized into two groups: the control group underwent normal saline eye drops, and the experimental group underwent Tripterygium Polyglucoside eye drops. After the chemical burns, the area of CNV was recorded and photographed, and the histology of cornea was evaluated under light microscopy. RESULTS: Fourteen and 28 days after creation of the model,the area of CNV in control group were 35.4±3.5mm² and 52.5±6.2mm², respectively; the area of CNV in experimental group was 30.0±2.7mm² and 41.0±5.6mm², respectively. There was significant difference between the two groups (P＜0.05). No obvious toxic effects of Tripterygium Polyglucoside eye drops to the corneal tissue were observed under the microscopy analysis. CONCLUSION: CNV can be significantly suppressed by the topical use of Tripterygium Polyglucoside eye drops.