AIM:To study the influence of holotrichia extractive to angiogenin1(Ang1)and pigment epithelium derivation factor(PEDF)expression and probe its inhibiting effect to the choroidal neovascularization(CNV)though establishing CNV model of experimental colored rabbits.

METHODS: Forty colored rabbits were selected and divided into 5 groups randomly, including blank group, model group, vitamin E group, zhujingwanjiajianfang group and the holotrichia extractive group. There were 8 rabbits in each group(16 eyes). CNV model was established through the method of argon laser photo-coagulation. Then optic fundus colored pictures were taken after 24 hours, 7, 14, 21 and 28 days, fluorescence fundus angiography(FFA)was performed after 7, 14, 21 and 28 days and optical coherence tomography(OCT)was performed after 14 and 28 days. Then the rabbits were divided into 2 parts each group randomly, and executed by aeroembolism after 14 and 28 days. Their eyes' posterior segment tissues were selected to carry on organized microtome section, hematoxylin and eosin staining and Ang1, PEDF immunohistochemistry staining to analyze depressant effect of holotrichia extractive to CNV.

RESULTS: Determination of Ang1 content expression showed, blank group's content was the least in all groups(P<0.05), the content of holotrichia extractive group and zhujingwanjiajianfang group were less than others in experimental groups(P<0.05), holotrichia extractive group's content was less than zhujingwanjiajianfang group's content, but there was no significant difference, holotrichia extractive group's content between the 14 days and the 28 days has significant difference(P<0.05). Determination of PEDF content expression showed, blank group's content was the highest in all groups(P<0.05), the content of holotrichia extractive group and zhujingwanjiajianfang group were higher than others in experimental groups(P<0.05), holotrichia extractive group's content was higher than zhujingwanjiajianfang group's content, but there was no significant difference, holotrichia extractive group's content between the 14 days and the 28 days had significant difference(P<0.05).

CONCLUSION: The holotrichia extractive has active influence for the high experiment of Ang1 in CNV of the experimental colored rabbits, it can intervene reduction of PEDF and protects retinal tissue, and thus it has depressant effect to CNV.