The cinobufotalin injection was independently developed by China, and this new second category drug has pharmacological effects of regulation of the immune system, inhibition of tumor cell proliferation and anti-hepatitis B virus. It has been widely used to treat malignant tumor advanced stage, chronic hepatitis B, epidemic hemorrhagic fever and clinical treatment of other diseases. In recent years, it is widely used in ophthalmology. Much progress has been made particularly in diseases such as keratitis, pterygium, etc. Exploring ophthalmology new therapeutic approach by the integrated treatment, making use of the advantage of the Traditional Chinese medicine will be the direction of ophthalmic clinical treatment and research in the future.