AIM: To construct recombination eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes.

METHODS:We amplified the target gene of shuttle vector PHMCMVTSHR289, conjugated the target gene and eukaryotic expression plasmid pcDNA3.1+, and accredited whether pcDNA3.1+/TSHR289 was connected or not by enzymatic digestion and sequencing. Cationic liposomes encapsulated the recombination plasmid pcDNA3.1+/TSHR289.

RESULTS: Recombination plasmid pcDNA3.1+/TSHR289 digested with enzyme HindIII and the fragment through 0.8% gel electrophoresis showed 512bp strip. Recombination plasmid pcDNA3.1+/TSHR289 were found synonymous mutation through forward(AAC to AAT)and reverse sequencing(GCG to GCT). The volume ratio of cationic liposomes and recombinant plasmid was 3:1.

CONCLUSION: It is successful to construct the recombination plasmid pcDNA3.1+/TSHR289 by accredit it through enzymatic digestion and sequencing.