AIM: To observe the accuracy and smooth degree of the matrix surface of the corneal donor in the laser ablation of the femtosecond laser.

METHODS: The FS200 laser cut 5 eye bank donors to get 5 anterior lamellar discs(ALD)and the corresponding 5 posterior lamellar discs(PLD). The thickness of PLD was not less than 150μm. The laser frequency was 200KHz. The lamellar cut energy was 1.2μJ and the trephination cut energy was 1.2μJ. The thickness of PLD, the histological changes of corneas(HE staining)and the degree of surface roughness under scanning electron microscope were recorded.

RESULTS: The corneal thickness before cutting was 534±24μm. The preset posterior lamellar corneal thickness was 199±7μm. The obtaining posterior lamellar thickness was 196±40 μm(Z=-0.13, P=0.89). With HE staining, the corneal cutting surface was slightly rough. Under the scanning electron microscopy, the smoothness score of the anterior lamellar stromal surface was 1.2±0.4, the posterior lamellar stromal surface smoothness score was 2.4±0.5(Z=-2.12, P=0.03).

CONCLUSION: The precision of deep corneal stroma cutting made by FS200 femtosecond laser is good. The cutting surface of the ALD is smoother than that of the PLD.