As one of the important contents of eyes, aqueous humor is always in dynamic circulation. When any part of the outflow is blocked, the aqueous humor outflow(AHO)circulation will break down, and followed by the increase of intraocular pressure, the damaged visual function, and the optic nerve injury. At last, the glaucoma occurs. Therefore, it can promote to locate the blocked part by observing the structure of the AHO and evaluating its function state, so as to clarify the pathogenesis of primary open angle glaucoma. Current imaging techniques for AHO pathway include optical coherence tomography(OCT)and angiography of outflow. This article will review the research progress of structure and function imaging of AHO.