To observe the effects of chromosome 10 (PTEN) and the tumor suppressor gene in proliferative LECs of the rabbit. ·METHODS: Forty-two white rabbits were randomly divided into test group (36 rabbits) and control group (6 rabbits). The transparent lenses of treated rabbits were operated with extracapsular cortex extraction, and the controls were kept untouched. The rabbits were sacrificed 1 day, 3 days, 1 week, 2 weeks, 1 month and 2 months after surgery. Immunohistochemistry was applied to detect proliferative cellular nuclei antigen (PCNA) as well as hybridization and reverse-transcriptase polymerase chain reactions (RT-PCR) were applied to detect phosphatase as well as tensin homology deleted on PTEN mRNA in lens equartor. ·RESULTS: PCNA expression increased significantly to a high level at 1 week, began to reduce 2 weeks later, and recovered to the normal level 1 month or 2 months after the surgery. PTEN mRNA expressed positively in normal rabbit LECs. The relative PTEN mRNA contents reduced greatly 1 day after operation and remained low level at 3 day. It began to increase slightly at 1 week, kept rising at 2 weeks and regained the normal expression after 1 month or 2 months. There was inverse correlation between the PTEN mRNA and PCNA expression. ·CONCLUSION: PTEN mRNA expresses positively in normal rabbit LECs plasma. PTEN participates in LECs proliferation and correlates with lens proliferative conditions