Home > Archive>Volume 12, Issue 2, 2019 >212-218. DOI:10.18240/ijo.2019.02.05
HTML XML Export Cite reminder
Attenuation of periostin in retinal Müller glia by TNF-α and IFN-γ
Author:
Corresponding Author:

Ye-Di Zhou. Department of Ophthalmology, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China. zhouyedi@csu.edu.cn

Fund Project:

Supported by National Natural Science Foundation of China (No.81800855; No.81800856; No.81700837); Natural Science Foundation of Hunan Province (No.2018JJ3765); Department of Science and Technology, Hunan (No.2015TP2007); Japan Society for the Promotion of Science KAKENHI Grants (No.26293374; No.16K15734).

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • | | |
  • Comments
    Abstract:

    AIM: To investigate the regulation and mechanisms of periostin expression in retinal Müller glia, and to explore the relevance to retinal neovascularization. METHODS: The oxygen-induced retinopathy (OIR) mouse model and the human Moorfield/Institute of Ophthalmology-Müller 1 (MIO-M1) cell line were used in the study. Immunofluorescence staining was used to determine the distribution and expression of periostin and a Müller glial cell marker glutamine synthetase (GS). Cytokines TNF-α and IFN-γ were added to stimulate the MIO-M1 cells. ShRNA was used to knockdown periostin expression in MIO-M1 cells. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was conducted to assess the mRNA expression of periostin. RESULTS: Immunofluorescence staining showed that periostin was expressed by MIO-M1 Müller glia. GS-positive Müller glia and periostin increased in OIR retinas, and were partially overlaid. The stimulation of TNF-α and IFN-γ reduced the mRNA expression of periostin significantly and dose-dependently in MIO-M1 cells. Knockdown of periostin reduced mRNA expression of vascular endothelial growth factor A (VEGFA) in MIO-M1 cells, while VEGFA expression was not changed in periostin knock-out OIR retinas. CONCLUSION: Müller glia could be one of the main sources of periostin in the retina, and might contribute to the pathogenesis of retinal neovascularization. Proinflammatory cytokines TNF-α and IFN-γ attenuate the periostin expression in retinal Müller glia, which provides a potential and novel method in treating retinal neovascular diseases.

    Reference
    Related
    Cited by
Get Citation

Ying-Qian Peng, Man-Jing Cao, Shigeo Yoshida,/et al.Attenuation of periostin in retinal Müller glia by TNF-α and IFN-γ. Int J Ophthalmol, 2019,12(2):212-218

Copy
Share
Article Metrics
  • Abstract:2477
  • PDF: 752
  • HTML: 329
  • Cited by: 0
Publication History
  • Received:July 09,2018
  • Revised:November 05,2018
  • Online: January 03,2019
You are the 4405072 Visitors
Governing Body：Department of Health, Provincial Government of Shaanxi, China
Organizer：Xi'an Medical Association of China
Address：Room 11707-08, Building 2, Unit 1, Wanda Plaza, No.8 Yanta North Road, Xi'an 710054, Shaanxi Province, China
Fax：(8629)82245172 Phone：(+8629)82245172/82210956
Email：ijopress@163.com
International Journal of Ophthalmology ® 2025 All Rights Reserved   The terms of CC BY-NC-ND 4.0 are applicable to all open access content.