AIM: To assess the prevalence of meibomian gland dysfunction (MGD) in staffs and faculty members of Sichuan University, China. METHODS: The records of the annually systemic physical examination of 4404 consecutive staffs and faculty members of Sichuan University were analyzed retrospectively. Ocular symptoms and signs of ocular surface were evaluated. RESULTS: MGD was diagnosed in 1424 participants (32.3%), with a mean age of 43.0±9.6y. Of these, 718 (50.4%) were females and no significant difference was found between males and females. The highest prevalence was found in the age 50-59y (36.0%). Logistic regression analysis showed that age is an impact factor of MGD (P<0.001, odds ratio=1.014). CONCLUSION: The prevalence of MGD in staffs and faculty members of a Chinese university is 32.3%, and increases with age.