Home > Archive>Volume 14, Issue 11, 2021 >1660-1665. DOI:10.18240/ijo.2021.11.03
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Increased cGAS/STING signaling components in patients with Mooren’s ulcer
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Xiao-Lin Qi. Eye Hospital of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250021, Shandong Province, China. qinglianqxl@163.com

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Supported by National Natural Science Foundation of China (No.81900907); the Young and Middle-Aged Scientists Research Awards Fund of Shandong Province (No.ZR2017BH004).

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    Abstract:

    AIM: To explore the expression of cGAS/STING signaling components in Mooren’s ulcer (MU). METHODS: Samples were obtained from ten MU patients, and eight residual corneal-scleral rings of healthy donor corneas for controls. Human corneal epithelial cells (HCECs) were used to evaluate the effect of cGAS/STING signaling pathway. Immunohistochemistry (IHC) and Western blot were used to examine the expression of cGAS, STING, and phosphorylated interferon regulatory factor 3 (p-IRF3) in MU tissues. The expression of interferon-β (IFN-β) and interferon-stimulated genes (ISGs) was quantified by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). RESULTS: The protein levels of cGAS and STING in MU samples were significantly elevated when compared with the healthy controls by Western blot and IHC. After stimulation with cGAMP, real-time PCR and ELISA showed a dramatic increase of IFN-β and ISGs (containing CXCL10, IFIT1, and IL-6) in HCECs. Moreover, HCECs treated with cGAMP was characterized by increased phosphorylation and more nuclear translocation of IRF3. Meanwhile, increased p-IRF3 was observed in MU samples via IHC and Western blot. CONCLUSION: The pronounced expression of cGAS/STING signaling components in the patients with MU and probably contribute to the onset and development of MU.

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Ya-Ni Zhang, Yan-Ling Dong, Wen-Pei Hao,/et al.Increased cGAS/STING signaling components in patients with Mooren’s ulcer. Int J Ophthalmol, 2021,14(11):1660-1665

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Publication History
  • Received:April 15,2021
  • Revised:August 05,2021
  • Online: October 27,2021
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