A novel approach for 25-gauge transconjunctival sutureless vitrectomy to evaluate vitreous substitutes in rabbits
Contact Author:

Ming Zhang. Department of Ophthalmology, West China Hospital, Sichuan University, 37 Guoxue Lane, Wuhou District, Chengdu 610041, Sichuan Province, China. zhangmingscu0905@163.com


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Supported by the Project of National Key Research and Development (No.2018YFC1106103); the Health Commission of Hubei Province Scientific Research Project (No.WJ2019Q024).

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    AIM: To improve the standard three-port vitrectomy for establishing and evaluating an endotamponade model in rabbits. METHODS: Three ports were prepared near the third eyelid of rabbits, and the infusion port was placed at the inferior nasal quadrant with the inserted cannula linking with a self-designed handheld rigid infusion catheter. All right eyes of rabbits underwent a modified 25-gauge vitrectomy and were subsequently filled with balanced salt solution, silicone oil, and eight-arm polyethylene glycols (8-arm PEGs) hydrogel separately for comparison. Ophthalmic examinations were performed regularly to record the changes after the surgery. RESULTS: Successful vitrectomy was achieved among 44 chinchilla rabbits. The mean operation time was 4.51±1.25min. Four eyes (9.1%) presented limited lens touch and two eyes (4.5%) showed retinal touch during surgery. Incision leakage was found in three eyes (6.8%) after surgery. There was no endophthalmitis, hemorrhage, or retinal detachment during the observation period and ophthalmic examinations after the implantation of vitreous substitutes. CONCLUSION: The modified technique of the standard vitrectomy applied in the endotamponade model in rabbits shows excellent safety and practicality.

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Rui-Jin Ran, Ting Wang, Meng-Ying Tao, et al. A novel approach for 25-gauge transconjunctival sutureless vitrectomy to evaluate vitreous substitutes in rabbits. Int J Ophthalmol 2023;16(10):1568-1573

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  • Received:March 02,2022
  • Revised:August 09,2023
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  • Online: September 19,2023
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