2009, 2(1):34-37.
Abstract:
AIM: To investigate the expression of EMMPRIN, MMP1,
MMP9 and TIMP2 in retinoblastoma (RB) and normal retinal
tissues and their clinicopathological significance and interrelationship.
METHODS: Envision immunohistochemistry stainings of
EMMPRIN, MMP1, MMP9 and TIMP2 were performed in 30
enucleated eyeballs with retinoblastoma and 15 specimens of
normal retina tissue, which had been routinely imbedded with
paraffin.
RESULTS: Positive rate of EMMPRIN, MMP1, MMP9 expression
was higher in RB tissue than in normal control ( P<0.01),
while TIMP2 expression was lower in RB than in normal retinal
tissue ( P<0.01). Samples from RB cases of clinical stage
I, differentiated type, and life span ≥ 2 years had lower positive
rate in expression of EMMPRIN, MMP1, MMP9 than those
from RB cases of clinical stage III, undifferentiated type, andP
life span<2 years ( P<0.05 or <0.01), while samples from
RB cases of differentiated type, optic nerve unaffected, and
life span ≥ 2 years had markedly higher positive rate in expression
of TIMP2 than those from RB cases of undifferentiated
type, optic nerve involved and life span<2 years ( P<0.05
or P<0.01). In RB tissues, EMMPRIN, MMP1, MMP9 expressions
were highly consistent ( P<0.05), whereas TIMP2 expression
is highly inconsistent with EMMPRIN,MMP1,MMP9
expression levels( P<0.05).
CONCLUSION: The expression level of EMMPRIN, MMP1,
MMP9 and TIMP2 may be an important marker of RB
progression, invasion and prognosis. There exist internally
mutual regulation relations among them."
