Volume 3,Issue 4,2010 Table of Contents

1 Effect of puerarin on retinal pigment epithelial cells apoptosis induced partly by peroxynitrite via Fas/FasL pathway

Li-Na Hao , Yan-Qing Zhang , Yu-Hua Shen , Zhi-Yun Wang , Yan-Hua Wang , Hai-Fang Zhang , Shou-Zhi He

2010, 3(4):283-287. DOI: 10.3980/j.issn.2222-3959.2010.04.01

[Abstract](1805) [HTML](0) [PDF 236.53 K](1107)

Abstract:
AIM: To evaluate the peroxynitrite (ONOO-) of puerarin on retinal pigment epithelial (RPE) cells apoptosis induced partly by peroxynitrite via Fas/FasL. METHODS: RPE cells from C57BL/6 mice eyes were cultured. Diabetes was induced in Sprague-Dawley (SD) rats by streptozotocin (STZ) intraperitoneal injection. Puerarin was administrated to cultured RPE cells and diabetic rats. Western blotting analysis, DNA ladder, RT-PCR, immunohistochemistry were used for determining the expression of nitrotyrosine (NT, the foot print of ONOO-), complement 3 (C3); apoptosis and inducible nitric oxide synthase (iNOS) mRNA as well as Fas/FasL signal transduction in RPE cells. RESULTS: Both RPE cells in ONOO- and puerarin group developed apoptosis and expressed NT, C3, iNOS mRNA and Fas/FasL. But latter delayed the all changes in a time-dependent manner compared with control and STZ group (P＜0.001). iNOS, C3 and Fas/FasL were up-regulated and associated with an increase of expression of ONOO- in vivo and in vitro. CONCLUSION: Puerarin decreases RPE cells apoptosis partly induced by ONOO- for diabetic retinopathy.

2 [Ca2+]i homeostasis and caspase-3 gene expression in verapamil-induced retinal pigment epithelium cells apoptosis in vitro

Dong-Bo Pang , Jing Hong

2010, 3(4):288-290. DOI: 10.3980/j.issn.2222-3959.2010.04.02

[Abstract](1840) [HTML](0) [PDF 126.81 K](1197)

Abstract:
AIM: To study caspase-3 gene expression and [Ca2+]i homeostasis in verapamil (Ver)-induced human retinal pigment epithelium (RPE) cells apoptosis. METHODS: Ver 80mg/L was applied in cultured human RPE cells for 12, 24 and 48 hours to induce RPE cells apoptosis. The expression of apoptotic effector gene caspase-3 was assessed by reverse transcription polymerase chain reaction (RT-PCR). Single cell was measured using fluorescence indicator Fura-3/AM with MetaFluo4.5/coolsnapfx/IX70 intracellular Ca2+ fluorescence imaging system. RESULTS: High levels of expression of caspase-3 mRNA were observed in normal RPE cells and it significantly increased after co-cultured with Ver. The fluorescence in resting RPE cells was strong and distributed throughout the cells. The nucleus appeared more fluorescent than the cytoplasm. Calcium fluorescence of RPE cells attenuated after co-cultured with Ver. CONCLUSION: Up-regulation of caspase-3 gene expression and disturbance of [Ca2+]i homeostasis might play pivotal roles in Ver-induced RPE cells apoptosis.

3 Optic nerve lesions in diabetic rats:blood flow to the optic nerve, permeability of micro blood vessels and histopathology

Jun-Ping Zhao , Zhi-Zhong Ma , Chen Song , Xiang-Hong Li , Yu-Zhen Li , Yu-Ying Liu

2010, 3(4):291-294. DOI: 10.3980/j.issn.2222-3959.2010.04.03

[Abstract](2321) [HTML](0) [PDF 199.47 K](1065)

Abstract:
AIM: To study optic nerve lesions, changes in blood flow to the optic nerve, and permeability of micro blood vessels and histopathology in diabetic rats. METHODS: Male Wistar rats (n=20) were randomly divided into control and diabetic groups. The diabetic model was prepared by a single injection of streptozotocin (50mg/kg) into the caudal vein. Three months later, laser Doppler perfusion imaging was used to observe the changes in blood flow to the optic nerve. Each rat was injected with 15g/L Evans blue (5μL/g). The permeability of microvessels in diabetic optic nerves was measured by spectrophotometry. Optic nerves were observed by light and transmission electron microscopy. RESULTS: Diabetic rats had atrophic optic nerve fibers with neurite swelling, loss of myelin, and a greater-than-normal proliferation of astrocytes, occurring within 3 months of induction of diabetes. Blood flow to the optic nerve was lower in diabetic rats than in controls. Microvessel permeability in diabetic rats increased 2.03-fold compared to controls. CONCLUSION: Diabetic rats develop significant pathological changes in the optic nerve, reduced blood flow to the optic nerve and increase microvessel permeability.

4 Inhibition of corneal neovascularization by vascular endothelia growth inhibitor gene

Hong Wang , Bing Wang

2010, 3(4):295-298. DOI: 10.3980/j.issn.2222-3959.2010.04.04

[Abstract](1817) [HTML](0) [PDF 890.75 K](976)

Abstract:
AIM: To evaluate the effect of EffecteneTM lipofectine mediated plasmids encoding human pcDNA4-vascular endothelia growth inhibitor (pcDNA4-VEGI) gene on corneal neovascularization (CNV). METHODS: Forty New Zealand albino rabbits were sutured by 5-0 silk on the superior cornea to induce CNV and divided into 4 random teams, ten per each team: team A: transfected by pcDNA4-VEGI gene mediated by EffecteneTM lipofectine transfection; team B: by plasmid pcDNA4; team C: by EffecteneTM, and team D: by normal saline. Length and area of CNV were observed under slit lamp every day after tran- sfection. Immunohistochemistry was performed to detect the expression of VEGI protein in corneas at day 3, 7, 14 and 21. RESULTS: 1) Average occurrence of CNV was 6.3 days in team A, 3.1 days in team B, 3.2 days in team C, and 3.2 days in team D. Difference was significant between A and other teams (P<0.01); 2) Length and average area of CNV in each period in team A was significantly different from those in team B, C and D (P<0.01); 3) VEGI expressions were observed in epithelium, stroma, endothelium and the cliff of CNV in team A at 3 days after transfection by immunohistochemical staining. None VEGI positive cells were found in the control teams (team B, C and D) all the time. CONCLUSION: EffecteneTM lipofectine transfection technique can effectively transfect pcDNA4-VEGI gene into rabbit cornea and the length and CNV areas can be inhibited by VEGI gene.

5 5-hydroxytryptamine level and 5-HT2A receptor mRNA expression in the guinea pigs eyes with spectacle lens-induced myopia

Ji-Wen Yang , Yan-Chun Xu , Lin Sun , Xiao-Dan Tian

2010, 3(4):299-303. DOI: 10.3980/j.issn.2222-3959.2010.04.05

[Abstract](2507) [HTML](0) [PDF 166.59 K](1101)

Abstract:
AIM: To investigate 5-hydroxytryptamine (5-HT) function and 5-HT receptor 2A (5-HT2A) mRNA expression in the formation of lens-induced myopia (LIM). METHODS: Lens-induced myopia construction method was applied to generate myopia on guinea pig right eye (LIM eye). RESULTS: LIM eyes formed significant myopia with longer axial length. 5-HT level in retina, choroids and sclera from LIM eyes was significantly higher than that in control group. 5-HT2A mRNA expression was also significantly up-regulated. CONCLUSION: Refraction lens could induce myopia in guinea pig and 5-HT may play an important role in the formation of myopia by binding with 5-HT2A receptor.

6 β-catenin expression in rat neovascularized cornea after alkali burn

Li Wang , Ming-Chang Zhang , Ying Zhang , Zhi He , Li Zhang , Shi-Yi Xiao

2010, 3(4):304-307. DOI: 10.3980/j.issn.2222-3959.2010.04.06

[Abstract](1903) [HTML](0) [PDF 179.73 K](983)

Abstract:
AIM: To investigate the expression of β-catenin in cornea after alkali burn and explore its role in cornea neovascularization (CNV). METHODS: CNV model was established by putting filter paper with the size of 3.0mm in diameter immersed in 1mol/L NaOH solution on the left cornea of rat for 20 seconds. Twenty-five Sprague Dawley rats were randomly divided into 5 groups: post-operation 1-, 4-, 7-, 14- and 21-day groups while the right eyes as normal control group. The expression level of β-catenin protein, mRNA and VEGF were determined at the 1st, 4th, 7th, 14th and 21st day following the establishment of model by RT-PCR and immunohistochemical technique. RESULTS: No expression of β-catenin immunoreactivity was detected in normal cornea. The expressions of β-catenin and VEGF both reached the peak at the 4th and 7th day and gradually decreased to near baseline 21 days later. Alteration of β-catenin and VEGF levels showed a significant positive correlation (r=0.855, P<0.05). CONCLUSION: The levels of β-catenin are markedly related to inflammatory CNV in rat cornea after alkali burn.

7 Ultrastructural pathology of corneal neovascu-larization after photodynamic therapy in rabbits

Hong Chen , Ming-Chang Zhang , Zhi Wang , Ying Zhang

2010, 3(4):308-310. DOI: 10.3980/j.issn.2222-3959.2010.04.07

[Abstract](1750) [HTML](0) [PDF 148.66 K](1046)

Abstract:
AIM: To investigate the ultrastructural pathogenesis of photodynamic therapy (PDT) for the experimental corneal neovascularization (CNV) by Hematoporphyrin Derivate (HPD) as photosensitizer and Argon laser as light source. METHODS: Experimental CNV models were induced in 7 white rabbits using alkali burn. Six weeks after models establishment, animals with CNV were injected with HPD intravenously, and 48 hours after the injection, 7 eyes were irradiated with argon laser (power 800mw, wavelength 514.5nm, spot diameter 200μm, exposure time 2ms). The irradiated CNV was observed by light microscopy and scanning electron microscopy. RESULTS: Histopathological study indicated that there was a striking decrease in the number of the CNV, vascular endothelium became degeneration and necrosis, some vessels were atrophy and attenuated, and vessels cavity were blocked by some thrombosis. No obvious abnormal histopathological findings were noted in surrounding tissues. CONCLUSION: The high precise action on CNV and minimal damage to surrounding tissues with PDT by HPD as photosensitizer suggested that PDT might be an effective and safe modality in the treatment of CNV.

8 Histological observation of RGCs and optic nerve injury in acute ocular hypertension rats

Shuang Li , Jia-Hua Fang , Fa-Gang Jiang

2010, 3(4):311-315. DOI: 10.3980/j.issn.2222-3959.2010.04.08

[Abstract](2477) [HTML](0) [PDF 215.60 K](1132)

Abstract:
AIM: To explore the injury of retinal ganglion cells (RGCs) and optic nerves in acute ocular hypertension (OHT) rats. METHODS: We retrogradely labeled RGCs and optic nerves of Sprague-Dawley rats by injecting 20g/L fluorogold (FG) into bilateral superior colliculi. Twenty-four hours after the injection, the right eyes were performed physiological saline anterior chamber perfusion with intraocular pressure maintained at 100mmHg for 60 minutes, while the contralateral eyes were performed sham procedure as control group without elevation of the saline bottle. Retinal hematoxylin and eosin (HE) sections, retinal whole mounts and frozen sections were made 14 days later to observe the morphology and survival of RGCs. Frozen sections and transmission electron microscopy were utilized to investigate the histological manifestations of optic nerves at the same time. RESULTS: A larger number of RGCs presented in control group. It had an average density of 1995±125/mm2 and distributed uniformly, while RGCs in OHT eyes reduced significantly to 1505±43/mm2 compared with control group (P<0.05). The optic nerves in control group showed stronger and more uniform fluorescence on the frozen sections, and the auxiliary fibers as well as myelin sheaths were in even and intact organization by transmission electron microscopy. However, exiguous fluorescence signals, vesicular dissociation and disintegration of myelin sheaths were found in OHT group. CONCLUSION: The present study suggested that fluorogold retrograde tracing is a feasible, convenient method for quantitative and qualitative study of neuronal populations and axonal injury in acute ocular hypertension rats.

9 Comparison of fibrin glue and suture technique in pterygium surgery performed with limbal autograft

Bora Yüksel , Sultan Kaya ünsal , Sevgi Onat

2010, 3(4):316-320. DOI: 10.3980/j.issn.2222-3959.2010.04.09

[Abstract](6216) [HTML](0) [PDF 165.03 K](1434)

Abstract:
AIM: To compare the efficiency and safety of fibrin glue to suture technique in pterygium surgery performed with limbal autograft. METHODS: A prospective randomised clinical trial was carried out in 58 eyes of 58 patients operated for primary nasal pterygium. Autologous conjunctival graft taken from the superotemporal limbus was used to cover the sclera after pterygium excision. In 29 eyes, the transplant was attached to the sclera with a fibrin tissue adhesive (Beriplast P) and in 29 eyes with 8-0 Virgin silk sutures. The Mann-Whitney U test was used for statistical analysis. Postoperative patient discomfort (pain, stinging, watering) and biomicroscopic findings (hyperemia, edema) were graded. Patients were followed up at least for six months. RESULTS: Subconjunctival hemorrhage occured under the graft in one patient in group 1. In seven cases of group 2, sutures were removed at the 15th day because of granulomatous tissue reaction. Patient symptoms were significantly less and biomicroscopic findings were better in group 1. Pterygium recurrence was seen in one case of group 1, and 2 cases of group 2. Average surgery cost was higher (P<0.05) and surgery time was shorter (P<0.05) in fibrin group. CONCLUSION: Using fibrin glue for graft fixation in pterygium surgery causes significantly less postoperative pain and shortens surgery time significantly.

10 Chronic macular edema associated with extrafoveal vitreoretinal traction

Aaron Trevino , Michael R Martinez , Avinoam Ophir

2010, 3(4):321-325. DOI: 10.3980/j.issn.2222-3959.2010.04.10

[Abstract](2127) [HTML](0) [PDF 242.17 K](1143)

Abstract:
AIM: To describe an association between extrafoveal vitreoretinal traction and chronic macular edema, either diffuse (DiME) or cystoid (CME), by the use of optical coherence tomography (OCT). METHODS: Charts and OCT findings of two patients with persistent DiME or persistent DiME accompanied by CME, both associated with extrafoveal vitreous traction membranes were analyzed. Excluded were eyes that either had another vitreoretinopathy that could affect the analysis, had undergone pars plana vitrectomy or that had been treated by intravitreal medications. An age-matched normal control group for OCT (n=12) allowed for the quantification of the normal macular thicknesses. RESULTS: One patient (one eye) following perforating ocular injury and one patient (one eye) of idiopathic origin, both with chronic macular edema refractive to conventional treatment, were found to be associated with extrafoveal vitreoretinal traction in each eye. Retinal edema that was underlying the traction site in each eye was in continuum with the central macular edema, thus manifesting as diffuse macular edema. The automatic central 6-radial lines program in the OCT enabled the detection of the traction site in one eye, while in the other eye the diagnosis was achieved only with the additional use of the Line group program. CONCLUSION: Chronic diffuse macular edema might be related to extrafoveal vitreoretinal traction. Careful search with the diverse OCT programs should be made in order to detect extrafoveal traction sites. Further studies and a larger cohort are required to compare the efficacy of early vitrectomy or pharmacologic vitreolysis versus the current therapeutic approaches in these situations.

11 Intraocular pressure as indicator of sympathetic asymmetry in the eyes

Sagili Chandrasekhara Reddy , Satagopam Mitti Mohan

2010, 3(4):326-327. DOI: 10.3980/j.issn.2222-3959.2010.04.11

[Abstract](2213) [HTML](0) [PDF 90.06 K](985)

Abstract:
AIM: To determine the asymmetry in the sympathetic activity in the eyes as indicated by intraocular pressure(IOP). METHODS: In a prospective cross sectional study, the IOP in 150 newborns, 80 young adults and 159 old people was measured with Tono-Pen under topical anaesthesia. RESULTS: The mean IOP in the newborns was 16.16mmHg in right eye and 15.79mmHg in left eye; in young adults 15.04mmHg in right eye and 14.71 in left eye; in old people 15.16 in right eye and 15.03 in left eye. A statistically significant higher IOP was noted in the right eye in the newborns (P=0.03) and in young adults (P=0.02), but not in the old people (P=0.26). The higher IOP in the right eye indicates the lowered sympathetic activity in that eye. CONCLUSION: We hypothesize that the sympathetic asy- mmetry in the bilaterally placed organs helps to establish the dominant pattern of the organ in the body.

12 Assessing amblyogenic factors in 100 patients with congenital ptosis

Abolfazl Kasaee , Alireza Yazdani-Abyaneh , Syed Ziaeddin Tabatabaie , Alireza K.Jafari , Ahmad Ameri , Bahram Eshraghi , Vafa Samarai , Meysam Mireshghi , Mohammad Taher Rajabi

2010, 3(4):328-330. DOI: 10.3980/j.issn.2222-3959.2010.04.12

[Abstract](2878) [HTML](0) [PDF 140.82 K](1095)

Abstract:
AIM: To study the frequency of amblyogenic factors in patients with congenital ptosis. METHODS: In this cross-sectional study, 114 eyes of 100 patients with congenital ptosis more than 1 year old were included. Amblyopia was defined as best-corrected visual acuity (BCVA) less than 10/10 or a difference between the two eyes of at least 2/10. In patients too young to be measured by the linear Snellen E test, fixation behavior was observed. Different types of amblyopia were assessed for each patient as: 1) anisometropic amblyopia: astigmatic anisometropia ≥1 dpt, hyperopic spherical anisometropia ≥1 dpt, myopic spherical anisometropia ≥-3 dpt (with cycloplegia); 2) strabismic amblyopia, and 3) stimulus deprivation amblyopia (SDA). Then the total incidence of amblyopia and each type of it were obtained. Patients with uni- and bi-lateral ptosis were also compared. RESULTS: The incidence of amblyopia in ptotic eyes was 39/114 (34.2 %), and for each specific cause was: refractive amblyopia in 29.8%, SDA in 10.5%, strabismic amblyopia in 4.3%. Amblyopia was more frequent in severe ptosis, 76% in patients with covered optical axes (OA), compared to non-covered OA (22.5%). In unilateral ptosis with covered OA, astigmatic anisometropic amblyopia was more frequent, and in bilateral ptosis with at least one eye covered OA, spherical anisometropic amblyopia was more frequent. In both unilateral and bilateral ptosis, SDA was more common if the OA was covered. CONCLUSION: As refractive anisometropic amblyopia is more prevalent than SDA, paying attention to all causes of amblyopia may be important in preventing amblyopia in a child with a ptotic eye.

13 Clinical spectrum of 15 patients with HIV-related ocular involvement in Tehran

Ali Abdollahi , Giv Heidari-Bateni , Reza Zarei , Parastou Kheirandish , Mohammadhosein Malekmadani , Minoo Mohraz , Maryam Abdollahi , Mohammad Taher Rajabi

2010, 3(4):331-336. DOI: 10.3980/j.issn.2222-3959.2010.04.13

[Abstract](1979) [HTML](0) [PDF 171.96 K](1064)

Abstract:
AIM: To determine the frequency of HIV-related ocular involvement and to describe the characteristics of involvement in a special clinic in Tehran. METHODS: In this cross sectional study, 141 patients (125 male and 16 female, 282 eyes) of HIV-infected patients with various stages of HIV infection that were referred to Center of behavioral diseases were evaluated during a period of 7 months. Every patient had a complete profile including demographic data, method of HIV transmission, recent CD4 T cell lymphocyte count, serological studies for common sexual or blood-born viruses and toxoplasmosis, history of antiretroviral therapy, and associated systemic disease. RESULTS: A total of 141 patients were evaluated. HIV-related ocular involvement was detected in 15 patients (10.6%), including 3 mycobacterium tuberculosis-related choroiditis, 2 cytomegalovirus retinitis, 2 retinal toxoplas- mosis, 2 herpes simplex virus-related lesions, 1 HIV- associated retinopathy, 1 herpes zoster ophthalmicus, 1 undetermined vitritis, and 3 cases of cranial nerve involvement including 2 cases of gaze palsy and 1 case of papilitis. In our study, mean CD4 T cell lymphocyte count was fewer in patients with ocular involvement than in patients without ocular involvement (204.7±123.8 vs 403.7±339.7, P=0.029), but there was no difference in other possible associated factors between two groups. CONCLUSION: Mycobacterium tuberculosis-related choroiditis and neurophthalmic lesions are the most common HIV-related ocular involvements in Tehran that is different from those of recent publications in developed countries.

14 Practical method to calculate post-LASIK corneal power: the Actual Ka+p method

Yi-Qian Hu , Xiang-Yu Ye , Xiu-Li Zhou , Yin Li , Cheng-Hui Xu , Lin-Lu Tian , Huang Zhu , Yao-Hua Sheng

2010, 3(4):337-341. DOI: 10.3980/j.issn.2222-3959.2010.04.14

[Abstract](2786) [HTML](0) [PDF 220.99 K](1386)

Abstract:
AIM: To evaluate the accuracy of a practical method (the Actual Ka+p method) of corneal power measurement for post-LASIK eyes undergoing cataract surgery. METHODS: Ten eyes of 7 patients (4 male, 3 female, average age 50.10±4.01 years, with -11.01±3.55D mean refraction before LASIK), underwent post-LASIK phaco+IOL cataract surgery. We used the posterior corneal curvature as measured by the Pentacam in a method we named Actual Ka+p to calculate the post-LASIK corneal power for IOL calculation. The refractive outcomes after cataract surgery were evaluated. The Actual Ka+p was compared with the back- calculated corneal power (BCK), which was thought to be the benchmark of true corneal power. The corneal power estimated by other published methods, including Maloney, Shammas, Koch-Maloney, Savini, and McCulley, together with the true net power and equivalent K reading (EKR) as found by the Pentacam were also compared with the BCK. RESULTS: All eyes achieved satisfied refractive status after cataract surgery. The difference between the postoperative refraction and the target refraction was 0.04±0.40D, range from -0.63D and +0.85D. Among all the methods we studied, although the Bonferroni multiple comparison tests did not detect significant differences between any two of them, the Actual Ka+p yielded the highest agreement with the BCK, with 80% of the eyes falling within ±0.5D and 100% within ±1.0D from the BCK values. CONCLUSION: The Actual Ka+p method can provide encour- aging results in post-LASIK eyes undergoing cataract surgery.

15 Management of morderate-to-severe Marcus-Gunn syndrome by anastomosis of levator and frontal muscles

Nan Xiang , Wei-Kun Hu , Bin Li , Rong Liu

2010, 3(4):342-345. DOI: 10.3980/j.issn.2222-3959.2010.04.15

[Abstract](3314) [HTML](0) [PDF 148.35 K](1960)

Abstract:
AIM: To study the effect of clinical management of moderate-to-severe Marcus-Gunn syndrome (MGS) by anastomosis of levator and frontal muscles. METHODS: The medical records of 13 patients with moderate-to-severe MGS who underwent surgeries in our institute between 2000 and 2007 were reviewed retrospectively. They underwent unilateral anastomosis of levator and frontal muscles under local anesthesia. RESULTS: Postoperative follow-up periods ranged from 6 to 36 months, with an average of 12 months. All eyelids (100%) showed complete resolution of jaw-winking, ten eyelids (76.9%) had good correction of ptosis, with equal plapebral apertures and symmetrical contours, three (23.1%) showed residual mild ptosis (<2mm). CONCLUSION: For moderate-to-severe MGS, unilateral ana- stomosis of levator and frontal muscles provides satisfied correction of jaw-winking and ptosis.

16 Prevention of exposure keratopathy in intensive care unit

Hua Shan , Du Min

2010, 3(4):346-348. DOI: 10.3980/j.issn.2222-3959.2010.04.16

[Abstract](5726) [HTML](0) [PDF 107.88 K](1307)

Abstract:
AIM: To compare the efficacy for preventing exposure keratopathy of three forms of eye care (artificial tear, moist chamber and polyethylene covers) for intensive care patients. METHODS: Eighty-four patients in Intensive Care Unit (ICU) were randomized to three treatment groups, including artificial tears group, moist chambers group and polyethylene film group. Patients of artificial tear group received two drops of carboxymethylcellulose drops to each eye every 2 hours. The moist chambers and the polyethylene were changed every 12 hours or as needed if they became unclean or torn. The corneal fluorescein stains were performed daily. RESULTS: No of 28 patients (0%) in the polyethylene group and one of the 27 patients (3.70%) in the moist chamber group had exposure keratopathy, compared to 8 of the 29 patients (27.59%) in the artificial tear group. There were statistical significance between the artificial tear group and the moist chamber group (P=0.02), and the artificial tear group and the polyethylene group (P =0.003). The time on eye care every day of the artificial tear group, the moist chamber group and the polyethylene group was 26.69±2.39 minutes, 35.33±2.63 minutes and 7.48±0.87 minutes, respectively. The eye care of the polyethylene group were statistically more time-save than that of the artificial tear group (P<0.001) and the moist chamber group (P<0.001). CONCLUSION: Polyethylene covers are more effective and more time-saving in reducing the incidence of corneal damage in intensive care patients

17 Alteration of anterior chamber in 81 glaucomatous eyes using Pentacam Scheimpflug system

Xuan Zou , Xuan-Chu Duan , Qian Zhong

2010, 3(4):349-351. DOI: 10.3980/j.issn.2222-3959.2010.04.17

[Abstract](2987) [HTML](0) [PDF 132.36 K](510)

Abstract:
AIM: To study the effects of gender, eye side and age on anterior chamber measurements; To compare the anterior chamber parameters between glaucoma and normal subjects. METHODS: A total of 169 patients (299 eyes), of which 122 normal subjects (218 eyes), 24 cases (39 eyes) with primary angle-closure glaucoma (PACG) and 22 cases (42 eyes) with primary open-angle glaucoma (POAG) visiting our hospital from Apr. 2008 to Oct. 2008 were enrolled. The parameters measured by Pentacam between different sexes, eye sides, ages and different groups were compared. RESULTS: The mean central anterior chamber depth (CACD), anterior chamber volume (ACV) and anterior chamber angle (ACA) measurements of normal eyes were 2.82±0.43mm, 159.74±43.64mm3 and 35.47±7.92°. There were no significant differences between different eye sides. There was a negative correlation between age and anterior chamber parameters (all P <0.001); The mean CACD, ACV and ACA were 1.87±0.29mm, 82.00±24.17mm3 and 21.94±7.11° in PACG group, and 2.67±0.34mm, 147.24±43.40mm3 and 31.19±6.34° in POAG group. The differences between PACG and age-matched normal subjects were significant (all P <0.05), but there was no significant difference between POAG and age-matched normal subjects (P >0.05). CONCLUSION: CACD, ACV and ACA decrease mildly with age. Anterior chamber parameters of PACG are smaller than that of normal eyes, but there is no difference between POAG and normal eyes.

18 Effectiveness of amniotic membrane transplantation combined with mitomycin C in the treatment of pterygium: a meta-analysis

Yin-Wei Song , Ai-Hua Yu , Xiao-Jun Cai

2010, 3(4):352-355. DOI: 10.3980/j.issn.2222-3959.2010.04.18

[Abstract](2173) [HTML](0) [PDF 192.56 K](1231)

Abstract:
AIM: To evaluate the recurrence rate and safety of amniotic membrane transplantation (AMT) augmented with mitomycin C (MMC) compared with amniotic membrane transplantation alone during the pterygium excision. METHODS: We took a meta-analysis on this program. Pertinent studies were selected through extensive searches of the Cochrane Library, MEDLINE, EMBASE, CBMdisc, CNKI. Pooled estimates were carried out in RevMan software v.4.2. RESULTS: Six trials reported postoperative recurrence rate of pterygium, included 882 eyes, three trials reported the complications. The results of meta-analysis showed that recurrence rate of AMT plus MMC group was 5.41%, AMT alone group was 16.89%, RR was 0.32, 95%CI ranged from 0.19 to 0.56, Z was 4.06, P <0.001. Two trials reported early complication as punctata keratitis, the incidence rate of AMT plus MMC group and AMT alone group were 17.14% and 0.00%, RR was 12.11, 95%CI ranged from 1.62 to 90.76. CONCLUSION: Amniotic membrane transplantation with MMC was associated with lower recurrence rate compared with amniotic membrane transplantation alone in pterygium excision，whether accompanied a higher risk with adverse events need more investigation.

19 New modified iris suture technique for pupillary dilation in aphakic eyes during vitreoretinal surgery

Feng-Mei Han , Quan-Hong Han , Yan-Hua Chu

2010, 3(4):356-357. DOI: 10.3980/j.issn.2222-3959.2010.04.19

[Abstract](2061) [HTML](0) [PDF 93.33 K](1038)

Abstract:
To describe a modified simple iris suture for pupillary dilation technique during vitrectomy in cases with a miotic pupil. Four translimbal incisions were created with a sharp straight blade at 1:30, 10:30, 4:30, and 7:30 o'clock, respectively. The straight needle of 10-0 polypropylene suture and a Sinskey IOL hook was used to displace the pupillary margin toward the limbus. In 3 cases, four sutures caused a 6-mm to 9-mm square-shaped pupil, and the pupil was allowed to return to a smaller size at the end of the operation. It is simple and may reduce postoperative complications.

20 Capsule contraction syndrome in Behcet’s disease

Ayse Gül Ko?ak Alintas , Derya Dal

2010, 3(4):358-360. DOI: 10.3980/j.issn.2222-3959.2010.04.20

[Abstract](2091) [HTML](0) [PDF 139.72 K](974)

Abstract:
We reported the case of a 39-year-old patient with inactive Behcet's syndrome developed capsule contraction syndrome after uneventful phacoemulsification and a foldable intraocular lens implantation. Two weeks after the operation, the patient was accepted to hospital with severe decrease of visual acuity (VA) on the operated eye. Capsule opening area had been reduced considerably by fibrotic tissue, which evaluated with full dilated pupil. Since capsule contraction syndrome (CCS) had been diagnosed in very early stage and which structure was not severely thick, the fibrotic band was treated successfully, 360 degree circular shape, by neodymium YAG (Nd:YAG) laser. Capsule opening area was remained stable during follow up period of one month. But a significant reocclusion was developed that need surgical reopening 3 months after initial operation. Continuous curvilinier capsulorhexis (CCC) may be recurred again after a successful treatment in patient with uveitis like Behcet's disease.

21 Syphilitic uveitis: report of 3 cases

Shin Wei Pan , Nor Sharina Yusof , Wan Hazabbah Wan Hitam , Raja Azmi Mohd Noor , Zunaina Embong

2010, 3(4):361-364. DOI: 10.3980/j.issn.2222-3959.2010.04.21

[Abstract](2949) [HTML](0) [PDF 163.70 K](1214)

Abstract:
AIM: To evaluate the clinical manifestations and visual outcome of syphilitic uveitis patients. METHODS: Case series of three patients with syphilitic uveitis who were managed in Hospital USM. RESULTS: Three patients were diagnosed to have uveitis secondary to Syphilis. All three patients were not known to have syphilis prior to presentation but have positive history of sexual promiscuity. All patients presented with progressive blurring of vision for average of one-month duration. Two of them have association with fever, ocular pain and floaters. Visual acuity at presentation ranges from 6/12 to hand movement. Mild anterior uveitis (non-granulomatous), vitritis and papillitis were presence in all the patients. First patient has multifocal chorioretinitis with exudative retinal detachment. The second patient presented with exudative retinal detachment while the third patient has chorioretinitis only. All the patients were treated with intramuscular benzyl-penicillin 2.4 MU weekly for 4 weeks and two of them received oral doxycycline 200mg twice daily for 3 months. The uveitis responded well to the treatment and two of them showed dramatic visual improvement from 6/120 to 6/21 and 6/12 to 6/6. The one with worse outcome was confirmed to have positive retroviral. CONCLUSION: Ocular syphilis presented here as non- granulomatous inflammation associated with exudative retinal detachment. Final visual outcome is generally good despite slow improvement after treatment.

22 New surgical therapy for conjunctival lymphan-giectasia

Jing Song , Ji-Feng Yu , Gai-Ping Du , Yi-Fei Huang

2010, 3(4):365-367. DOI: 10.3980/j.issn.2222-3959.2010.04.22

[Abstract](2222) [HTML](0) [PDF 114.99 K](1040)

Abstract:
AIM: To report 7 cases of conjunctival lymphangiectasia, introduce a simple operation method and discuss the result of the pathological examination. METHODS: Clinical data including medical history, physical examination, previous surgery history were collected in 7 patients with conjunctival lymphangiectasia. Ocular data including visual acuity, Slit-lamp biomicroscopy were released, as were results of histopathology studies. Surgical method was also introduced by video. RESULTS: Seven eyes of 7 patients (4 male and 3 female) with biopsy-proven conjunctival lymphangiectasia underwent the resection. Despite different surgical intervention, the optima method has been founding to treatment conjunctival lymphangiectasia. It is to reduce recurrence that the most concerned problem. In these 7 cases reported, patients kept healthy for 18 months without any one recurrence, whose visual acuity were not affected. Pathological report showed cystic tissue consists of squamous epithelium with significant hyperplasia. CONCLUSION: This operation method is simple and easy to complete, as it is deserved to accept for surgeons and patients on the basis of its advantages.

23 Aniridia rings implantation for treatment of congenital aniridia combined with cataract surgery: a case report

Juan-Juan Li , Yun-Peng Li , Zhu-Lin Hu

2010, 3(4):368-369. DOI: 10.3980/j.issn.2222-3959.2010.04.23

[Abstract](5794) [HTML](0) [PDF 87.46 K](1390)

Abstract:
We describe the successful treatment in a patient with bilateral congenital aniridia and cataract by insertion of capsular tension rings and IOL.

24 Congenital membranous cataract associated with persistent fetal vasculature

Zong-Duan Zhang , Li-Jun Shen , Jia Qu

2010, 3(4):370-371. DOI: 10.3980/j.issn.2222-3959.2010.04.24

[Abstract](3242) [HTML](0) [PDF 134.64 K](1229)

Abstract:
A 15-year-old boy underwent phacoemulsification for a membranous cataract during which the presence of anterior persistent hyaloid artery and elongated ciliary processes were discovered. A posterior capsulorhexis was performed and the anterior part of the persistent hyaloid artery stalk was resected together with the posterior capsule. A foldable intraocular lens was implanted and the optic was captured in the posterior capsulorhexis margin. This is an unusual case of congenital membranous cataract associated with malfor- mations of persistent fetal vasculature and elongated ciliary processes.

Home

Articles

Journal Info

For Authors

For Reviewers

Publication Policies

News and Events

RSS

Volume 3,Issue 4,2010 Table of Contents