Effect of d-timolol and l-timololon rat experimental choroidal neovascularization in vivo and endothelial cells in vitro
DOI:
Author:
Corresponding Author:

Affiliation:

Clc Number:

Fund Project:

Effectof D-Timolol and L-Timololon rat exPerimental choroidal neovascularization in vivo and endothelial cells in vitro . Int JoPhthalmol

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    ImPairmentof choroidal Perfusion was found in age-related macular degeneration(AMD) Patients. We Postulatedthat vasoactive agents, which can reduce choroidal blood flow resistance, might Preventthe develoPmentof choroidal neovascularization (CNV). D-Timolol and L-Timolol are hyPotensive agents used in cardiovascular and glaucomatheraPy.their effectson laser-induced exPerimental CNV rat model and human umbilical vein endothelial cells (HUVEC) werethus evaluated. ·METHODS: Male Brown Norway rats were anesthetizedto receive Nd:YAG laserto breakthe Bruch's membrane. D-Timolol and L-Timolol were givenonce dailythrough intraPeritoneal injection after lasertreatment for 4 weeks. Fluorescein angiograPhy(FA) was Performedon 2 weeks and 4 weeks. HUVEC weretested by Proliferation assay and adhesion assay with D-Timolol and L-Timolol at different concentrations. ·RESULTS: D-Timolol reducedthe fluorescein leakageto 83%ofthe control grouP in laser-induced rat's CNV model at a dosageof 15mg/(kg·d). L-Timolol had no effecton CNV formation even at a higher dosageof 20mg/(kg·d). D-Timolol inhibitedthe endothelial cells Proliferation significantly by 300mg/L. L-Timolol also significantly inhibitedthe cell Proliferation at 1 000mg/L. But at a lower dose such as 300mg/L, no significant inhibitory effect was found. Both drugs showed no effecton cell adhesion function in cell culture exPeriments. ·CONCLUSION: D-Timolol was foundto Prevent CNV develoPment in laser-induced model in vivo and inhibit vascular endothelial cells Proliferation in vitro . L-Timolol had no effecton cell Proliferation atthe same dose, and neitheron rat CNV model.the results indicatethesetwo isomers have different functionson rat's CNV Prevention andon HUVEC cell Proliferation.

    Reference
    Related
    Cited by
Get Citation

Xin-Rong Xu, Yan-Hong Zou, George C Y Chiou. Effect of d-timolol and l-timololon rat experimental choroidal neovascularization in vivo and endothelial cells in vitro. Int J Ophthalmol, 2008,1(3):208-211

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:July 21,2008
  • Revised:August 15,2008
  • Adopted:
  • Online:
  • Published: