Quantitative detection of the expression level of transforming growth factor -β1 and -β2 in rat retina with real time PCR
DOI:
Author:
Corresponding Author:

Affiliation:

Clc Number:

Fund Project:

National Natural Science Foundation of China (No. 30271391); Foundation of Scientific Research Program of Health Bureau of Shanghai City, China (No. 034124); Foundation of Training Plan for a hundred Trans-century Excellent Subject Leaders in Shanghai Public Health System, China (No. 057)

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To quantitatively detect the expression level of transforming growth factor-β1 (TGF-β1) and transforming growth factor-β2 (TGF-β2) genes in the retina of normal rat in order to determine the expression difference of TGF-β1 and TGF-β2 in retina. METHODS: The total RNA was isolated from which the first strand of cDNA was prepared. The mRNA levels of TGF-β1 and TGF-β2 were detected quantitatively by real time polymerase chain reaction (PCR). RESULTS: The mRNA levels of TGF-β1 and TGF-β2 were 0.0008±0.0003 and 0.0378±0.009, respectively. Expression of TGF-β2 was obviously higher than that of TGF-β1 in rat retina with statistical significance (t =12.37, P <0.001).The ratio of TGF-β2/TGF-β1 was 55.00±26.61. CONCLUSION: Quantitative reverse transcription polymerase chain reaction (QRT-PCR) can specifically and accurately detect gene expression level in rat retina. In retina the TGF-β2 gene is expressed more abundantly than TGF-β1. It is suggested that TGF-β2 play an important role in retina diseases.

    Reference
    Related
    Cited by
Get Citation

Wei Shen, Lin Liu. Quantitative detection of the expression level of transforming growth factor -β1 and -β2 in rat retina with real time PCR. Int J Ophthalmol, 2009,2(4):316-319

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:August 30,2009
  • Revised:November 12,2009
  • Adopted:
  • Online:
  • Published: