Preliminary findings of immunological characteristics in primary human corneal endothelial cells
DOI:
Author:
Corresponding Author:

Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To evaluate the function of primary human corneal endothelial cells (HCEC) serving as immunological cells. METHODS: Expression of HLA-DP, -DQ, -DR, CD40, CD80, and CD86 was determined by immunohistochemical methods. Meanwhile, purified peripheral blood mononuclear cells(PBMC) were cocultured with primary HCEC which were pre-treated with and without γ -IFN respectively. The activation of lymphocytes was determined by FACS analysis. RESULTS: In coculture system, T lymphocyte was activated by primary HCEC, HLA-DP, -DQ, -DR and CD40 expression were increased by γ -IFN induction. Costimulatory molecular CD80 was shown on the endothelial cells. CONCLUSION: Primary HCEC are assumed to be involved in the corneal transplantation rejection process as potential antigen presenting cells (APC).

    Reference
    Related
    Cited by
Get Citation

Fan Wang, Bai-Lin Song, Hong-Yang Guo. Preliminary findings of immunological characteristics in primary human corneal endothelial cells. Int J Ophthalmol, 2009,2(4):328-330

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:September 18,2009
  • Revised:October 22,2009
  • Adopted:
  • Online:
  • Published: