AIM: To compare the measurement of anterior chamber depth (ACD) inclusive of corneal thickness using intraocular lens (IOL) master and ultrasound biomicroscopy (UBM) and evaluate the repeatability of each method. METHODS: Two consecutive measurements of ACD were prospectively performed using IOL master and UBM in 60 eyes in 60 individuals. Mean values were compared using the paired t test. For each individual, ACD measurements was performed 5 times to estimate the repeatability of each method by a coefficient of variation (CV). RESULTS: The mean ACD was 2.95?.25mm with the IOL master and 2.96±0.22mm with the UBM. This difference was not statistically significant (P =0.631). The coefficient of variation (CV) was 0.56%±0.26% and 0.65%±0.36% in IOL master and UBM, respectively. CONCLUSION: The mean ACD of IOL master is the same as UBM. The repeatability of IOL master is better than UBM.