AIM: To evaluate the effect of the application of sodium hyaluronate on the operation of suturing the corneal perforation. METHODS: Sodium hyaluronate was used in the operation of suturing the corneal perforation in 76 patients to form the anterior chamber, protect eye tissues and separate the anterior iris synechia. RESULTS: In the procedure of operation, the anterior chamber was stable, the prolapsed iris was easily restored, the corneal wound was easily sutured and there was no damage to the lens. Postoperatively, the corneal wound was closed well, the anterior chamber formed and there was no anterior iris synechia. Fifty-four cases had round pupils and the pupils of 22 cases were not round with defect of iris. CONCLUSION: Sodium hyaluronate in the operation of suturing the corneal perforation can help form the anterior chamber, protect the eye tissues and make the suturing corneal wound easier. It can reduce the rate of complication and promote the wound healing.