Inhibited experimental corneal neovascularization by neutralizing anti-SDF-1α antibody
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Supported by National Natural Science Foundation in China (NSFC No. 30771978 and No 30972712); Qing-Lan Project of Education Bureau of Jiangsu Province, and Supported by Jiangsu Province’s Key Provincial Talents Program, China(No. RC2011104)

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    Abstract:

    AIM: To explore the effect of SDF-1α on the development of experimental corneal neovascularization (CRNV). METHODS: CRNV was induced by alkali injury in mice. The expression of SDF-1α and CXCR4 in burned corneas was examined by Flow Cytometry. Neutralizing anti-mouse SDF-1α antibody was locally administrated after alkali injury and the formation of CRNV 2 weeks after injury was assessed by Immunohistochemistry. The expression of VEGF and C-Kit in burned corneas was detected by RT-PCR. RESULTS: The number of CRNV peaks at 2 weeks after alkali injury. Compared to control group, SDF-1α neutralizing antibody treatment significantly decreased the number of CRNV. RT-PCR confirmed that SDF-1α neutralizing antibody treatment resulted in decreased intracorneal VEGF and C-Kit expression. CONCLUSION: SDF-1α neutralizing antibody treated mice exhibited impaired experimental CRNV through down regulated VEGF and C-Kit expression.

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Gao-Qin Liu, Pei-Rong Lu, Long-Biao Li, et al. Inhibited experimental corneal neovascularization by neutralizing anti-SDF-1α antibody. Int J Ophthalmol, 2012,5(1):7-12

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