Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells
Author:
Corresponding Author:

Affiliation:

Clc Number:

Fund Project:

The Foundation for Young Talents of Gansu Province, China (No. 1208RJYA013)

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM:To analyze proteomic and signal transduction alterations in irradiated melanoma cells.METHODS:We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interaction was used to analyze relationships among proteins.RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells.CONCLUSION:Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.

    Reference
    Related
    Cited by
Get Citation

Lu-Bin Yan, Kai Shi, Zhi-Tong Bing, et al. Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells. Int J Ophthalmol, 2013,6(3):286-294

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:November 26,2012
  • Revised:May 06,2013
  • Adopted:May 06,2013
  • Online: June 24,2013
  • Published: