Effects of transforming growth factor β2 and connective tissue growth factor on induction of epithelial mesenchymal transition and extracellular matrix synthesis in human lens epithelial cells
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National Natural Science Foundation of China (No.81070721); International Exchange Program of Shaanxi Province, China (No.2012KW-31)

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    Abstract:

    AIM:To investigate the effects of transforming growth factorβ2 (TGF-β2) and connective tissue growth factor (CTGF) on transdifferentiation of human lens epithelial cells (HLECs) cultured in vitro and synthesis of extracellular matrix (ECM).METHODS:HLECs were treated with TGF-β2 (0, 0.5, 1.0, 5, 10μg/L) and CTGF (0, 15, 30, 60, 100μg/L) for different times (0, 24, 48, 72h) in vitro and the expression of α-smooth muscle actin (α-SMA), the main component of the extracellular matrix type I collagen (Col-1) and fibronectin (Fn) were measured by using real-time polymerase chain reaction (PCR) and western-blot.RESULTS: TGF-β2 and CTGF significantly increased expression of α-SMA mRNA and protein (P<0.05, P<0.001), Fn mRNA and protein (P<0.001), Col-1 mRNA and protein (P<0.001). TGF-β2 could induce HLECs expression of CTGF mRNA and protein in dose-dependent manner (P<0.05, P<0.001). TGF-β2 and CTGF could induce HLECs to express α-SMA, Fn and Col-1 in time-dependent manner. Each time of TGF-β2 and CTGF induced HELCs expression of α-SMA, Fn, Col-1 mRNA and protein was significant increase compared with control (P<0.05, P<0.001).CONCLUSION: TGF-β2 and CTGF could induce HLECs epithelial mesenchymal transition and ECM synthesis.

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Cheng Pei, Bo Ma, Qian-Yan Kang, et al. Effects of transforming growth factor β2 and connective tissue growth factor on induction of epithelial mesenchymal transition and extracellular matrix synthesis in human lens epithelial cells. Int J Ophthalmol, 2013,6(6):752-757

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Publication History
  • Received:September 23,2013
  • Revised:October 14,2013
  • Adopted:October 14,2013
  • Online: December 22,2013
  • Published: