Hypoxia increases adipogenesis and affects adipocytokine production in orbital fibroblasts–a possible explanation of the link between smoking and Graves’ ophthalmopathy
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1Department of Endocrinology, Singapore General Hospital, Level 3, the Academia, 20 College Road 169856, Singapore;
2Department of Clinical Research, Academia Discovery Tower Level 9 Research Office 2, 20 College Road 169856, Singapore;
3Oculoplastic/Aesthetic Eyeplastic Service, Singapore National Eye Centre, 11 Third Hospital Avenue 168751, Singapore

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Supported by Endocrinology and Metabolic Society of Singapore.
Conflicts of Interest: Chng CL, None; Lai OF, None; Chew CS, None; Peh YP, None; Fook-Chong SM, None; Seah LL, None; Khoo DH, None.

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    Abstract:

    AIM: To assess the effects of hypoxia on human orbital fibroblasts (OF) on adipogenesis and adipocytokine production.METHODS:Human OF were derived from tissues obtained from patients with Graves’ ophthalmopathy (GO) and from patients without known thyroid diseases undergoing blepharoplasty. The OF were cultured separately under normoxic and hypoxic conditions. Comparisons of adipocytokine concentrations using multiplex ELISA and lipid accumulation in the cells using Oil Red O staining were subsequently performed.RESULTS:There was increased adipogenesis in OF from GO subject when exposed to hypoxic culture conditions. This was not observed in OF from normal controls. Hypoxia led to an increase in leptin and a decrease in MCP-1 secretion in OF cultures.CONCLUSION: Hypoxia induces adipogenesis in OF and may represent a mechanism by which smoking contributes to deterioration of GO. We also found novel changes to leptin and MCP-1 production in OF cultures exposed to hypoxia suggesting important roles of these cytokines in the disease process.

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Chiaw Ling Chng, Oi Fah Lai, Charmaine Sze-Min Chew, et al. Hypoxia increases adipogenesis and affects adipocytokine production in orbital fibroblasts–a possible explanation of the link between smoking and Graves’ ophthalmopathy. Int J Ophthalmol, 2014,7(3):403-407

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Publication History
  • Received:January 13,2014
  • Revised:February 25,2014
  • Adopted:February 25,2014
  • Online: June 24,2014
  • Published: