Transforming growth factor-β2 induces morphological alteration of human corneal endothelial cells in vitro
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(Department of Ophthalmology, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China)

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Supported by National High Technology Research and Development Program (“863” Program) of China (No.2006AA02A132); Key Developing Discipline of Hebei Province (No.201221) .
Conflicts of Interest: Wang J, None; Fan TJ, None; Yang XX, None; Chang SM, None.

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    Abstract:

    AIM:To investigate the morphological altering effect of transforming growth factor-β2 (TGF-β2) on untransfected human corneal endothelial cells (HCECs) in vitro.METHODS: After untransfected HCECs were treated with TGF-β2 at different concentrations, the morphology, cytoskeleton distribution, and type IV collagen expression of the cells were examined with inverted contrast light microscopy, fluorescence microscopy, immunofluorescence or Western Blot.RESULTS:TGF-β2 at the concentration of 3-15 μg/L had obviously alterative effects on HCECs morphology in dose and time-dependent manner, and 9 μg/L was the peak concentration. TGF-β2 (9 μg/L) altered HCE cell morphology after treatment for 36h, increased the mean optical density (P<0.01) and the length of F-actin, reduced the mean optical density (P<0.01) of the collagen type IV in extracellular matrix (ECM) and induced the rearrangement of F-actin, microtubule in cytoplasm and collagen type IV in ECM after treatment for 72h. CONCLUTION:TGF-β2 has obviously alterative effect on the morphology of HCECs from polygonal phenotype to enlarged spindle-shaped phenotype, in dose and time-dependence manner by inducing more, elongation and alignment of F-actin, rearrangement of microtubule and larger spread area of collagen type IV.

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Jing Wang, Ting-Jun Fan, Xiu-Xia Yang, et al. Transforming growth factor-β2 induces morphological alteration of human corneal endothelial cells in vitro. Int J Ophthalmol, 2014,7(5):759-763

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Publication History
  • Received:December 19,2013
  • Revised:June 13,2014
  • Adopted:June 13,2014
  • Online: October 20,2014
  • Published: