Effect of titanium dioxide nanoparticles on zebrafish embryos and developing retina
Author:
Corresponding Author:

Affiliation:

Clc Number:

Fund Project:

Foundations: Supported by the National Natural Science Foundation of China (No.81301080); the National Key Technology R&D Program of China (2012BAI08B06); the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.
Conflicts of Interest: Wang YJ, None; He ZZ, None; Fang YW, None; Xu Y, None; Chen YN, None; Wang GQ, None; Yang YQ, None; Yang Z, None; Li YH, None.

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM:To investigate the impact of titanium dioxide nanoparticles (TiO2 NPs) on embryonic development and retinal neurogenesis. METHODS:The agglomeration and sedimentation of TiO2 NPs solutions at different dilutions were observed, and the ultraviolet-visible spectra of their supernatants were measured. Zebrafish embryos were experimentally exposed to TiO2 NPs until 72h postfertilization (hpf). The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization. RESULTS: The1 mg/L was determined to be an appropriate exposure dose. Embryos exposed to TiO2 NPs had a normal phenotype. The neurogenesis was initiated on time, and ganglion cells, cones and rods were well differentiated at 72 hpf. The expression of fms mRNA and the 4C4 antibody, which were specific to microglia in the central nervous system (CNS), closely resembled their endogenous profile. CONCLUSION:These data demonstrate that short-term exposure to TiO2 NPs at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.

    Reference
    Related
    Cited by
Get Citation

Ya-Jie Wang, Zi-Zi He, Yang-Wu Fang, et al. Effect of titanium dioxide nanoparticles on zebrafish embryos and developing retina. Int J Ophthalmol, 2014,7(6):917-923

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:May 14,2014
  • Revised:July 03,2014
  • Adopted:July 03,2014
  • Online: December 15,2014
  • Published: