Anti-apoptosis effects of vascular endothelial cadherin in experimental corneal neovascularization
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Supported by the National Natural Science Foundation of China (No. 81200727; No. 30972712); Jiangsu Province’s Key Provincial Talents Program (No. RC2011104); Suzhou Municipal Natural Science Foundation (No. SYS201448); the Soochow Scholar Project of Soochow University.

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    Abstract:

    AIM: To explore the effects and mechanism of vascular endothelial cadherin (VE-cadherin) on experimental corneal neovascularization (CRNV). METHODS: Mouse corneas were burned with sodium hydroxide to build a CRNV model. The burned corneas were locally administrated with anti-mouse VE-cadherin neutralizing antibody. Annexin V and cluster of differentiation 31 (CD31) double staining was used to measure vascular endothelial cell apoptosis with the use of flow cytometry (FCM). The protein expression of NADPH oxidase 2 (Nox2), caspase-3, and protein kinase C (PKC) in the burned corneas were examined by Western blot. Human retinal endothelial cell (HREC) proliferation was detected using a Cell Counting Kit 8 (CCK-8) assay in vitro. RESULTS: The amount of CRNV peaked two weeks after the alkali burn. FCM confirmed that VE-cadherin neutralizing antibody treatment increased CD31 positive cell apoptosis. Western blot revealed that the intracorneal protein expression of Nox2 and caspase-3 were up-regulated, while PKC was down-regulated in the VE-cadherin neutralizing antibody administrated group. CCK-8 assay showed that VE-cadherin neutralizing antibody markedly inhibited HREC proliferation. CONCLUSION: VE-cadherin exhibited an anti-apoptosis effect through enhanced PKC signaling and an enhanced cell proliferation pathway.

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Gao-Qin Liu, Hong-Ya Wu, Jing Xu, et al. Anti-apoptosis effects of vascular endothelial cadherin in experimental corneal neovascularization. Int J Ophthalmol, 2015,8(6):1083-1088

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Publication History
  • Received:December 18,2014
  • Revised:April 13,2015
  • Adopted:
  • Online: November 12,2015
  • Published: