Nitric oxide and tumour necrosis factor alpha in the process of pseudoexfoliation glaucoma
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Tatjana S. Sarenac Vulovic. Department of Ophthalmology, Clinical Center Kragujevac, Zmaj Jovina 30, Kragujevac 34000, Serbia. tvoja.tanja@yahoo.com

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    Abstract:

    AIM: To establish the role of nitric oxide (NO), ascorbic acid and tumour necrosis factor-α (TNF-α) in the pathogenesis of pseudoexfoliation glaucoma (XFG). METHODS: Our study included 120 patients who were referred for cataract surgery. All patients were divided into four groups according to clinical findings: XFG, early and late pseudoexfoliation syndrome (XFS), and cataract (without pseudoexfoliation). Serum and aqueous humour levels of the ascorbic acid, NO and TNF-α were measured. The concentrations of the ascorbic acid and NO were measured by an appropriate spectrophotometric method. Enzyme-linked immunosorbent assay (ELISA) was used to determine TNF-α level. RESULTS: Aqueous humour concentration of ascorbic acid was significantly lower in patients with late XFS (0.61±0.11 mmol/L) and XFG (0.48±0.15 mmol/L) compared to patients with early XFS (0.9±0.15 mmol/L) and cataract (1.16±0.22 mmol/L), while there was no difference in serum concentration in all examined groups. Aqueous humour concentration of NO was significantly higher in patients with XFG (77.7±11.4 μmol/L) compared to patients with early XFS (50.27±9.34 μmol/L) and cataract (49.77±7.1 μmol/L), while serum concentration was increased in the early stage of XFS (73.26±8.29 μmol/L). Aqueous humour level of proinflammatory cytokine TNF-α was increased in patients with XFS (early 460.04±18.32 pg/mL; late 502.42±53.23 pg/mL) and XFG (510.34±43.07 pg/mL), while there was no difference in serum level in all examined groups of patients. CONCLUSION: Reduced ascorbic acid and elevated NO and inflammation related cytokine TNF-α level in aqueous humour of the patients with developed XFG suggest that oxidative stress induces local inflammation.

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Tatjana S. Sarenac Vulovic, Sladjana M. Pavlovic, Vladimir LJ. Jakovljevic, et al. Nitric oxide and tumour necrosis factor alpha in the process of pseudoexfoliation glaucoma. Int J Ophthalmol, 2016,9(8):1138-1142

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Publication History
  • Received:July 19,2015
  • Revised:October 30,2015
  • Adopted:
  • Online: August 15,2016
  • Published: