The role of LOX-1 on innate immunity against Aspergillus keratitis in mice
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Gui-Qiu Zhao. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, No.16 of Jiangsu Road, Shinan District, Qingdao 266003, Shandong Province, China. yankeyuelihui@126.com

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Supported by the National Natural Science Foundation of China (No.81470609; No.81170825); Key Project of Natural Science Foundation of Shandong Province (No.ZR2012HZ001); Specialized Research Fund for the Doctoral Program of Higher Education (No.20123706110003); Youth Project of Natural Science Foundation of Shandong Province (No.ZR2013HQ007).

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    Abstract:

    AIM: To explore the effects of lectin-like ox-LDL receptor (LOX-1) on innate immunity against Aspergillus fumigatus (A. fumigatus ) in mice cornea. METHODS: The mRNA levels of LOX-1 were tested in normal and A. fumigatus infected corneas of C57BL/6 and BALB/c mice. The expression of LOX-1, pro-inflammatory cytokines TNF-α, CXCL1 and IL-6, anti-inflammatory cytokines IL-10, and matrix metalloproteinase 9 (MMP9) were tested with treatment with LOX-1 neutralizing antibody or control IgG in A. fumigatus infected corneas of C57BL/6. Macrophages and neutrophils were extracted from susceptible C57BL/6 mice, and pretreated with LOX-1 neutralizing antibody or IgG, then stimulated with A. fumigatus. The mRNA levels of LOX-1, TNF-α, CXCL1, IL-6, IL-10 and MMP9 were evaluated by polymerase chain reaction. RESULTS: The expression of LOX-1 was significantly increased in C57BL/6 mice corneas after A. fumigatus infection compared with BABL/c mice. After treatment with LOX-1 neutralizing antibody, the expression of LOX-1, TNF-α, CXCL1, IL-6, MMP9 and IL-10 in C57BL/6 corneas were significantly decreased compared with treatment with control IgG; the expression of LOX-1, CXCL1, IL-6 and IL-10 were significantly decreased in macrophages, while TNF-α and MMP9 expressions had no change; LOX-1, TNF-α, CXCL1, IL-6, MMP9 and IL-10 expressions were significantly decreased in neutrophils. CONCLUSION: The expression of LOX-1 can affect the expression of pro-inflammatory and anti-inflammatory cytokines in fungal infected corneas, macrophages and neutrophils of C57BL/6. LOX-1 inhibition rebalances the inflammatory response of fungal keratitis in mice.

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Kun He, Li-Hui Yue, Gui-Qiu Zhao, et al. The role of LOX-1 on innate immunity against Aspergillus keratitis in mice. Int J Ophthalmol, 2016,9(9):1245-1250

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Publication History
  • Received:January 28,2016
  • Revised:April 26,2016
  • Adopted:
  • Online: September 12,2016
  • Published: