Expression and regulation of microRNA-29a and microRNA-29c in early diabetic rat cataract formation
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Zhi-Jian Li. Department of Ophthalmology, First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin, Heilongjiang Province, China. lzj6515@sina.com

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    Abstract:

    AIM: To determine the role of microRNA (miRNA)-29a and miRNA-29c in the regulation of apoptosis in early rat diabetic cataract formation. METHODS: Streptozotocin (STZ)-induced diabetic Sprague-Dawley (SD) rats were used in the study. The expression level of miRNA-29a, miRNA-29c, and BCL2-modifying factor (BMF) in lens epithelial cells (LECs) samples were measured using quantitative real-time polymerase chain reaction. Prediction algorithms of miRanda, TargetScan 6.2, and mirRDB to perform a miRNA gene network analysis were used for the potential miRNA-29a and miRNA-29c targets. RESULTS: The miRNA-29a and miRNA-29c expression levels were all significantly lower in the control group compared to the 2 and 4wk diabetic samples (P<0.01). The network analysis indicated that one miRNA-29a and miRNA-29c targets was BMF. There was significantly higher expression of BMF mRNA compared to the normal controls (P<0.01). CONCLUSION: Apoptosis occurs in rat LECs following high blood glucose exposure. It is likely that apoptosis during diabetic cataract formation involves the decreased expression of miRNA-29a and miRNA-29c and the increased expression of BMF.

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Ying Sun, Chun-Mei Lu, Zhen Song, et al. Expression and regulation of microRNA-29a and microRNA-29c in early diabetic rat cataract formation. Int J Ophthalmol, 2016,9(12):1719-1724

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Publication History
  • Received:March 02,2016
  • Revised:May 11,2016
  • Adopted:
  • Online: December 07,2016
  • Published: