Evaluation of antioxidants and argpyrimidine in normal and cataractous lenses in north Indian population
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Bharani K Mynampati. Department of Ophthalmology, Advanced Eye Center, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India. bharanima@gmail.com

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Supported by Indian Council of Medical Research.

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    Abstract:

    AIM: To assess the level of glutathione, thioltransferase, and argpyrimidine in nuclear and cortical cataractous lenses as well as in the clear lenses in the north Indian population. METHODS: Human cataractous lenses were collected from the patients who underwent extracapsular cataract extraction surgery; clear lenses were collected from the freshly donated eye bank eyes. Antioxidant molecules such as glutathione and thioltransferase enzyme activity were measured; simultaneously in these lenses a blue fluorophore argpyrimidine, an advanced glycation end (AGE) product level was assessed using high performance liquid chromatography (HPLC). RESULTS: The protein concentration was found to be present at higher levels in the control lenses compared to cataract lenses. A significant decrease in the glutathione level was observed in the nuclear cataractous lenses compared to cortical cataractous (P=0.004) and clear lenses (P≤0.005), but no significant change in the level of antioxidant enzyme thioltransferase was observed. Further, argpyrimidine a blue fluorophore (AGE) was found to be significantly higher in the nuclear cataract (P=0.013) compared to cortical cataract lenses. CONCLUSION: Antioxidants such as glutathione significantly decrease in age-related nuclear and cortical cataract and an AGE, argpyrimidine are present at significantly higher levels in nuclear cataract.

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Bharani K Mynampati, Sujata Ghosh, Thungapathra Muthukumarappa, et al. Evaluation of antioxidants and argpyrimidine in normal and cataractous lenses in north Indian population. Int J Ophthalmol, 2017,10(7):1094-1100

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Publication History
  • Received:November 02,2016
  • Revised:January 19,2017
  • Adopted:
  • Online: July 11,2017
  • Published: