Differentiation of human olfactory mucosa mesenchymal stem cells into photoreceptor cells in vitro
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Ming Lu. Department of Neurosurgery, the 163rd Hospital of Chinese PLA, the Second Affiliated Hospital of Hunan Normal University, 1 Hongshan Bridge, Changsha 410003, Hunan Province, China. lumingcs163@163.com; Min-Li Huang. Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning 530021, Guangxi Zhuang Autonomous Region, China. nnhml@163.com

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Supported by Guangxi Natural Science Foundation (No.2014GXNSFAA118273); University Scientific Research Projects in Education Department of Guangxi Zhuang Autonomous Region (No.YB2014072).

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    Abstract:

    AIM: To investigate whether the human olfactory mucosa mesenchymal stem cells (OM-MSCs) can differentiate into photoreceptor cells in vitro. METHODS: Through the olfactory mucosa adherent method, olfactory mucosa was isolated, cultured and identified in vitro among mesenchymal stem cells. The cell surface markers were analyzed by flow cytometry, induced to differentiate into retinal photoreceptor cells in vitro, and the expression of rhodopsin was observed and identified by Immunofluorescence and Western blot methods. RESULTS: OM-MSCs from human were spindle cell-based, and showing radial colony arrangement. OM-MSCs were negative for CD34, CD45 and CD105, but positive for CD73 and CD90. Following induction, a strong positive reaction was produced by photoreceptor specific marker rhodopsin in the cells.

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Wen Lu, Da Duan, Zacharia Ackbarkhan, et al. Differentiation of human olfactory mucosa mesenchymal stem cells into photoreceptor cells in vitro. Int J Ophthalmol, 2017,10(10):1504-1509

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Publication History
  • Received:August 15,2016
  • Revised:August 01,2017
  • Adopted:
  • Online: September 30,2017
  • Published: