Suppression of corneal neovascularization by curcumin via inhibition of Wnt/β-catenin pathway activation
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Li Qin and Jing-Ming Li. Department of Ophthalmology, First Affiliated Hospital of Xi’an Jiaotong University, 277 Yantaxi Road, Xi’an 710061, Shaanxi Province, China. eyeqinli@163.com; Jingming_li@126.com

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Supported by Young Talent Research Scholar Grant (No.2016KJXX-12); Shaanxi Natural Science Grant (No.2016JM8029).

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    Abstract:

    AIM: To investigate whether curcumin suppressed corneal neovascularization (CNV) formation via inhibiting activation of Wnt/β-catenin pathway. METHODS: Suture-induced CNV was established on Sprague-Dawley (SD) rats. Curcumin were daily administrated by subconjunctival injection. Phosphorylation of low-density lipoprotein receptor-related protein 6 (LRP6) and nuclear accumulation of β-catenin, two indicators of activated Wnt/β-catenin pathway, were determined by Western-blot analysis in subconfluent/proliferating human microvascular endothelial cells (HMEC) and neovascularized corneas. Wnt3a conditioned medium (WCM) were harvested from Wnt3a expressing cells. WCM-induced cell proliferation and endothelial tubular formation capacity was measured by MTT assay and Matrigel assay, respectively. RESULTS: Phosphorylation of LRP6 and nuclear accumulation of β-catenin was significantly increased in subconfluent/proliferating endothelial cells. Activation of Wnt/β-catenin pathway by WCM markedly promotes HMEC proliferation and tubular formation. Curcumin inhibited LRP6 phosphorylation and nuclear accumulation of β-catenin. In addition, curcumin attenuated WCM-induced HMEC proliferation and disrupted tubular structure of endothelial cells on Matrigel. Meanwhile curcumin suppressed suture-induced CNV and inhibited LRP6 phosphorylation as well as β-catenin accumulation in SD rats. CONCLUSION: Taken together, activation of Wnt/β-catenin pathway could be involved in endothelial proliferation during suture-induced CNV formation and curcumin attenuated CNV formation via inhibition of Wnt/β-catenin pathway activation.

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Yong-Kang Zhang, Jing-Ming Li, Li Qin. Suppression of corneal neovascularization by curcumin via inhibition of Wnt/β-catenin pathway activation. Int J Ophthalmol, 2017,10(12):1791-1797

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Publication History
  • Received:April 07,2016
  • Revised:November 09,2016
  • Adopted:
  • Online: December 07,2017
  • Published: