Home > Archive>Volume 11, Issue 2, 2018 >189-195. DOI:10.18240/ijo.2018.02.02
HTML XML Export Cite reminder
Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells
Author:
Corresponding Author:

Yu-Wen Hsu. Department of Optometry, Da-Yeh University, No.168, University Rd., Dacun, Changhua 51591, Taiwan, China. yuwen@mail.dyu.edu.tw; Fung-Jou Lu. Institute of Medicine, College of Medicine, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd., Taichung City 40201, Taiwan, China. fjlu@csmu.edu.tw

Fund Project:

Supported by Ministry of Science and Technology, Taiwan (No.NSC 106-2314-B-212-004).

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    AIM: To investigate the anti-fibrosis effect of rosmarinic acid (RA) in pterygium epithelial cells (PECs) to determine if RA is a potent agent for treating pterygium. METHODS: The PECs (1×104 cells/mL) were treated with 100 μmol/L of RA for 1, 3 and 6h. After RA treatment, the cell viability was determined by staining with acridine orange/DAPI and analysis via a NucleoCounter NC-3000. The protein expression levels of type I collagen, transforming growth factor beta-1 (TGF-β1), TGF-β type II receptor (TGF-βRII), p-Smad1/5, p-Smad2, p-Smad3, and Smad4 of the cell lysates were measured by Western blot analysis. RESULTS: The cell viability of PECs was significantly decreased after RA treatment (P<0.01). As the result, RA reduced the protein expression of type I collagen and TGF-β1 of PECs. Additionally, RA also inhibited TGF-β1/Smad signaling by decreasing the protein expressions of TGF-βRII, p-Smad1/5, p-Smad2, p-Smad3, and Smad4. CONCLUSION: This study demonstrate that RA could inhibit fibrosis of PECs by down-regulating type I collagen expression and TGF-β1/Smad signaling. Therefore, RA is a potent therapeutic agent for the treatment of pterygium.

    Reference
    Related
    Cited by
Get Citation

Ya-Yu Chen, Chia-Fang Tsai, Ming-Chu Tsai,/et al.Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells. Int J Ophthalmol, 2018,11(2):189-195

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:June 23,2017
  • Revised:November 20,2017
  • Online: February 18,2018
You are the  Visitors
Governing Body：Department of Health, Provincial Government of Shaanxi, China
Organizer：Xi'an Medical Association of China
Address：Room 11707-08, Building 2, Unit 1, Wanda Plaza, No.8 Yanta North Road, Xi'an 710054, Shaanxi Province, China
Fax：(8629)82245172 Phone：(+8629)82245172/82210956
Email：ijopress@163.com
International Journal of Ophthalmology ® 2025 All Rights Reserved   The terms of CC BY-NC-ND 4.0 are applicable to all open access content.