Crosslink between lipids and acute uveitis: a lipidomic analysis
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Qian Sun. Department of Ophthalmology, Shanghai First People’s Hospital Affiliated to Jiaotong University, Shanghai 200080, China. Dr_sunqian@126.com

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Supported by the National Natural Science Foundation of China (No.81300776); the Youth Scientific Research Project of Shanghai Municipal Health Bureau (No.20134Y088).

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    Abstract:

    AIM: To explore the roles of phospholipids and sphingolipids in the inflammatory process of uveitis. METHODS: Aqueous humor (AH) and the retina were obtained from endotoxin-induced uveitis (EIU) rats during the acute inflammation stage (24h after endotoxin injection). Lipids were extracted using a modified Bligh and Dyer method and subjected to mass spectrometric identification using class-specific lipid standards and ratiometric quantification. Relative intensity analysis was performed to evaluate the amount change of common lipids between the EIU and control groups. RESULTS: Unique lipid species encompassing all five phospholipid classes were found in both control and the EIU AH and retina. Commensurate with the significantly increased level of lysophospholipids in the EIU AH and retina, we found that the ratio of lysophospholipids to total phospholipids was significantly increased too. We also detected a significant increase in 18:0 lysophosphatidylcholine levels in the EIU group (fold change =6.4 in AH and 3.8 in retina). Cer240, Cer241, and SM240 levels remarkably increased in the EIU AH. Enhanced C12 ceramide-1-phosphate (C12 C-1-P), C16 C-1-P, C24 C-1-P, and upregulated Cer160, Cer240, SM120, and SM240 were found in EIU retina. C-1-P was believed to restore homeostasis by inhibiting nuclear factor kappa B (NF-κB) activation. However, we still found elevated NF-κB levels in the EIU retina. CONCLUSION: A variety of lipids might have played a critical role in EIU inflammation. Exogenous topical application of these protective lipids or inhibition of these pro-inflammatory lipids may be useful therapeutic strategies for the resolution of EIU.

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Hai-Yan Wang, Yi Wang, Yuan Zhang, et al. Crosslink between lipids and acute uveitis: a lipidomic analysis. Int J Ophthalmol, 2018,11(5):736-746

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Publication History
  • Received:February 26,2018
  • Revised:March 15,2018
  • Adopted:
  • Online: May 11,2018
  • Published: