A preliminary study on the establishment of an animal model of conjunctivochalasis
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Qing-Song Li. Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, 164 Lanxi Road, Shanghai 200062, China. 13651602344@163.com

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Supported by the Key Medical Discipline Project of Shanghai Municipal Health Bureau-Ophthalmology (No.ZK2015A20); the Health System Independent Innovation Science Foundation of Shanghai Putuo District (No.2015PTKW001); Plateau Science, Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine.

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    Abstract:

    AIM: To explore a feasible method on the establishment of an animal model of conjunctivochalasis (CCH). METHODS: Twelve clean-grade New Zealand white rabbits were divided into four groups (n=3/group): the control group (one received no interventions, and the others underwent subconjunctival injection of sterile water), the matrix metalloproteinases (MMPs) group (administered subconjunctival injection of MMP-3), the aging group (administered subcutaneous injection of D-galactose), the tumor necrosis factor-α (TNF-α) solution group (administered eye drops of TNF-α). Anterior segment photography, conjunctival tissue light microscopy and transmission electron microscopy (TEM) were performed after 12wk. RESULTS: Among all groups, the MMPs group had the following changes: the looser connection between the inferior bulbar conjunctiva and sclera; the more disordered collagen fibers (Trichrome staining) and the broken elastic fibers (Aldehyde-fuchsin staining); the focal necrosis of fibroblasts (TEM). CONCLUSION: Administration of MMPs may be a feasible method for the establishment of an animal model of CCH.

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Jing-Yun Gan, Qing-Song Li, Huan-Ming Zhou, et al. A preliminary study on the establishment of an animal model of conjunctivochalasis. Int J Ophthalmol, 2018,11(6):899-904

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Publication History
  • Received:February 26,2018
  • Revised:April 08,2018
  • Adopted:
  • Online: June 12,2018
  • Published: