Early expression of PTX3 in Aspergillus fumigatus infected rat cornea
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Gui-Qiu Zhao. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China. zhaoguiqiu_good@126.com

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Supported by National Natural Science Foundation of China (No.81170825; No.81470609); Specialized Research Fund for the Doctoral Program of Higher Education (No.20123706110003); the Youth Natural Science Foundation of Shandong Province (No.ZR2013HQ007); the Key Project of Natural Science Foundation of Shandong Province (No.ZR2012HZ001).

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    Abstract:

    AIM: To investigate the expression of pentraxin 3 (PTX3) in rat corneal epithelium at the early stage of Aspergillus fumigatus (A. fumigatus) infection. METHODS: A total of 50 Wistar rats were randomly divided into control group, Sham group and experimental group (fungal keratitis group, FK group). The right eye was chosen as the experiment one and infected by A. fumigatus. Rats were executed at 8, 16 and 24h after the experimental models being established. Corneal epithelia were collected to assess the expression of PTX3 by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. RESULTS: Corneal inflammation scores increased as infection prolonged (P<0.05, P<0.001). PTX3 mRNA expression was low in normal and Sham group rats' corneas. Level of PTX3 mRNA in infected rat cornea was elevated at 8h and peaked at 16h. The difference was significant compared with control group (P<0.001). Western blot analysis also showed a significant increase of PTX3 protein in experimental group at 8h and peaked at 16h (P<0.001). The synchronous expression of control group and experimental group were also in significant difference (P<0.001). CONCLUSION: PTX3 exists in cornea epithelium and is significantly increased after A. fumigatus infection. PTX3 plays an important role in the early stage of cornea innate immunity against A. fumigatus.

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Jie Zhang, Gui-Qiu Zhao, Jing Qu, et al. Early expression of PTX3 in Aspergillus fumigatus infected rat cornea. Int J Ophthalmol, 2018,11(7):1084-1089

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Publication History
  • Received:February 22,2018
  • Revised:April 12,2018
  • Adopted:
  • Online: July 10,2018
  • Published: