Protection of tight junction between RPE cells with tissue factor targeting peptide
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Yu-Ping Zou. Department of Ophthalmology, General Hospital of Guangzhou Military of PLA, Guangzhou 510010, Guangdong Province, China. gzzouyuping@163.com

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Supported by Science and Technology Project of Guangzhou City (No.2014J4100035); the Project of the Third Affiliated Hospital of Guangzhou Medical University (No.2013Y06).

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    Abstract:

    AIM: To investigate the effect of tissue factor targeting peptide (TF-TP) on retinal pigment epithelium (RPE) cells tight junctions. METHODS: Cell counting kit-8 (CCK-8) was used to measure the proliferation of ARPE-19 cells. Expression of tight junction, ZO-1 in ARPE-19 cells was measured by Western blot and immunofluorescent staining. Western blot was also used to detect the expression of tissue factor (TF). CEC Transmigration Assay was used to measure the migration of ARPE-19 cells. The transport of fluorescent markers [fluorescein isothiocyanate dextrans of 4, 10, 20 (FD4, FD10, FD20)] and the transepithelial electrical resistance (TEER) were used to measure in ARPE-19 cell. RESULTS: CCK-8 assay showed that 5 μmol/L TF-TP can inhibit ARPE-19 cells abnormally proliferation stimulated by lipopolysaccharide (LPS; P<0.05). LPS increased the transport of fluorescent markers (FD4, FD10, FD20) and decreased TEER levels in ARPE-19 cells, respectively, which were prevented by 5 μmol/L TF-TP pretreatment (P<0.05). Furthermore, LPS significantly up-regulated the expression of TF and downregulated the expression of ZO-1 (P<0.05) in ARPE-19 cell which was inhibited by the TF-TP (P<0.05). In addition, TF-TP inhibited the abnormal migration induced by LPS in ARPE-19 cell (P<0.05). CONCLUSION: Our findings suggest that TF-TP suppressed proliferation and migration of ARPE-19 cells induced by LPS, and maintained the RPE tight junctions through inhibition of TF expression and increased expression of ZO-1.

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Xiu-Lan Zou, Guan-Feng Wang, Dan-Dan Li, et al. Protection of tight junction between RPE cells with tissue factor targeting peptide. Int J Ophthalmol, 2018,11(10):1594-1599

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Publication History
  • Received:March 10,2018
  • Revised:August 01,2018
  • Adopted:
  • Online: September 04,2018
  • Published: