Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs
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Hong Wang. Department of Ophthalmology, Qilu Hospital of Shandong University, No.107, Wenhuaxi Road, Lixia District, Jinan 250001, Shandong Province, China. hongwang123456@yeah.net

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Supported by Qilu Hospital of Shandong University (No.201805049).

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    Abstract:

    AIM: To investigate the effects of green flickering light on refractive development and expression of muscarinic acetylcholine receptor (mAChR) M1 in the eyes of guinea pigs. METHODS: Thirty guinea pigs (15-20 days old) were randomly divided into three groups (n=10/group). Animals in group I were raised in a completely closed carton with green flickering light illumination. Those in group II were kept in the open top closed carton under normal natural light. Guinea pigs were raised in a sight-widen cage under normal natural light in group III. The refractive status and axial length were measured before and after 8 weeks' illumination. Moreover, total RNA extracted from retinal, choroidal, and scleral tissues were determined by real-time reverse transcription polymerase chain reaction (RT-PCR). The expressions of the receptor M1 were also explored in the retina, choroid, and sclera using immunohistochemistry. RESULTS: There was a remarkable reduction in refractive error and increase in axial length after 8-weeks' green flickering light stimulation (P<0.001). The expression of M1 receptor mRNA in sclera and retina in myopia group were remarkably lower than that in group II and III (P<0.01). Significant reduced expression of M1 receptor stimulated by green flickering light in retina and sclera tissues were also observed (P<0.05). However, there was no M1 receptor expression in choroid in 3 groups. CONCLUSION: Myopia can be induced by 8 weeks' green flickering light exposure in the animal model. M1 receptor may be involved causally or protectively in myopia development.

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Yuan Tao, Xiao-Li Li, Li-Yuan Sun, et al. Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs. Int J Ophthalmol, 2018,11(11):1755-1760

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Publication History
  • Received:April 09,2018
  • Revised:August 22,2018
  • Adopted:
  • Online: November 07,2018
  • Published: