Home > Archive>Volume 12, Issue 2, 2019 >194-200. DOI:10.18240/ijo.2019.02.02
PDF HTML XML Export Cite reminder
Conjunctival microbiome changes associated with fungal keratitis: metagenomic analysis
Author:
Corresponding Author:

Yu-Sen Huang. Shandong Eye Institute, 5 Yanerdao Road, Qingdao 266071, China. Huang_yusen@126.com

Fund Project:

Supported by the National Natural Science Foundation of China (No.81670839); the Key Research and Development Plan of Shandong Province (No.2018CXGC1205).

  • Article
    • | |
  • Metrics
    • |
  • Reference [27]
    • |
  • Related [20]
    • | | |
  • Comments
    Abstract:

    AIM: To investigate the ocular surface microbiome profile of patients with fungal keratitis (FK) through bacterial 16S rDNA sequencing. METHODS: The swab samples were collected from 8 patients with FK (Group 1 from the corneal ulcer, Group 2 from the conjunctival sac of the infected eyes, and Group 3 from the conjunctival sac of the fellow eyes) and 10 healthy eyes (Group 4 from the conjunctival sac). Bacterial 16S rDNA V4-V5 region sequencing was performed to characterize the bacterial communities on the ocular surfaces of the patients with FK. RESULTS: Our metagenomic data showed that 97% of the sequence reads were categorized into 245 distinct bacterial genera, with 67.75±7.79 genera detected in Group 1, 73.80±13.44 in Group 2, 74.57±14.14 in Group 3, and 89.60±27.49 in Group 4. Compared with the healthy eyes (Group 4), both infected (Groups 1 and 2) and fellow eyes (Group 3) of the patients with FK showed reduced bacterial diversity and altered ocular surface microbiota compositions, with lower abundance of Corynebacterium and Staphylococcus and higher abundances of Pseudomonas, Achromobacter, Caulobacter and Psychrobacter. CONCLUSION: Our report depicts the altered ocular surface bacterial community structures both in the affected and fellow eyes of patients with FK. These changes may contribute to the pathogenesis of FK or the increased risk for FK.

    Reference
    1 Dan J, Zhou QJ, Zhai HL, Cheng J, Wan L, Ge C, Xie LX. Clinical analysis of fungal keratitis in patients with and without diabetes. PLoS One 2018;13(5):e0196741.
    2 Wen XF, Miao L, Deng YH, Bible PW, Hu X, Zou YL, Liu Y, Guo SX, Liang JR, Chen TT, Peng GH, Chen W, Liang LY, Wei L. The influence of age and sex on ocular surface microbiota in healthy adults. Invest Ophthalmol Vis Sci 2017;58(14):6030-6037.
    3 Kugadas A, Christiansen SH, Sankaranarayanan S, Surana NK, Gauguet S, Kunz R, Fichorova R, Vorup-Jensen T, Gadjeva M. Impact of microbiota on resistance to ocular pseudomonas aeruginosa-induced keratitis. PLoS Pathog 2016;12(9):e1005855.
    4 Kugadas A, Gadjeva M. Impact of microbiome on ocular health. Ocul Surf 2016;14(3):342-349.
    5 Kugadas A, Wright Q, Geddes-McAlister J, Gadjeva M. Role of microbiota in strengthening ocular mucosal barrier function through secretory IgA. Invest Ophthalmol Vis Sci 2017;58(11):4593-4600.
    6 de Paiva CS, Jones DB, Stern ME, Bian F, Moore QL, Corbiere S, Streckfus CF, Hutchinson DS, Ajami NJ, Petrosino JF, Pflugfelder SC. Altered mucosal microbiome diversity and disease severity in sjögren syndrome. Sci Rep 2016;6:23561.
    7 Lee SH, Oh DH, Jung JY, Kim JC, Jeon CO. Comparative ocular microbial communities in humans with and without blepharitis. Invest Ophthalmol Vis Sci 2012;53(9):5585-5593.
    8 Zhang HK, Zhao FX, Hutchinson DS, Sun WF, Ajami NJ, Lai SJ, Wong MC, Petrosino JF, Fang JH, Jiang J, Chen W, Reinach PS, Qu J, Zeng CQ, Zhang DK, Zhou XT. Conjunctival microbiome changes associated with soft contact lens and orthokeratology lens wearing. Invest Ophthalmol Vis Sci 2017;58(1):128.
    9 Naik S, Bouladoux N, Linehan JL, Han SJ, Harrison OJ, Wilhelm C, Conlan S, Himmelfarb S, Byrd AL, Deming C, Quinones M, Brenchley JM, Kong HH, Tussiwand R, Murphy KM, Merad M, Segre JA, Belkaid Y. Commensal-dendritic-cell interaction specifies a unique protective skin immune signature. Nature 2015;520(7545):104-108.
    10 Fan D, Coughlin LA, Neubauer MM, Kim J, Kim MS, Zhan XW, Simms-Waldrip TR, Xie Y, Hooper LV, Koh AY. Activation of HIF-1α and LL-37 by commensal bacteria inhibits Candida albicans colonization. Nat Med 2015;21(7):808-814.
    11 Tanure MA, Cohen EJ, Sudesh S, Rapuano CJ, Laibson PR. Spectrum of fungal keratitis at wills eye hospital, philadelphia, pennsylvania. Cornea 2000;19(3):307-312.
    12 St Leger AJ, Desai JV, Drummond RA, Kugadas A, Almaghrabi F, Silver P, Raychaudhuri K, Gadjeva M, Iwakura Y, Lionakis MS, Caspi RR. An ocular commensal protects against corneal infection by driving an interleukin-17 response from mucosal γδ T cells. Immunity 2017;47(1):148-158.e5.
    13 Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol 1998;5(10):R245-R249.
    14 Huang Y, Yang B, Li W. Defining the normal core microbiome of conjunctival microbial communities. Clin Microbiol Infect 2016;22(7): 643.e7-643643.e12.
    15 Yang BX, Li H, Kong FF, Huang YS. Identification of pathogenic bacteria in aqueous and vitreous of endophthalmitis by 16S rDNA sequencing technique. Chin J Exp Ophthalmol 2016;34(10):883-887.
    16 Mago? T, Salzberg SL. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 2011;27(21):2957-2963.
    17 Dong QF, Brulc JM, Iovieno A, Bates B, Garoutte A, Miller D, Revanna KV, Gao X, Antonopoulos DA, Slepak VZ, Shestopalov VI. Diversity of bacteria at healthy human conjunctiva. Invest Ophthalmol Vis Sci 2011;52(8):5408.
    18 Krishnan T, Sengupta S, Reddy PR, Ravindran RD. Secondary pseudomonas infection of fungal keratitis following use of contaminated natamycin eye drops: a case series. Eye (Lond) 2009;23(2):477-479.
    19 Ligon JM, Hill DS, Hammer PE, Torkewitz NR, Hofmann D, Kempf HJ, van Pée KH. Natural products with antifungal activity from Pseudomonas biocontrol bacteria. Pest Manag Sci 2000;56(8):688-695.
    20 Dirksen P, Marsh SA, Braker I, Heitland N, Wagner S, Nakad R, Mader S, Petersen C, Kowallik V, Rosenstiel P, Félix MA, Schulenburg H. The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host-microbiome model. BMC Biol 2016;14:38.
    21 Justesen US, Holt HM, Thiesson HC, Blom J, Nielsen XC, Dargis R, Kemp M, Christensen JJ. Report of the first human case of caulobacter sp. infection. Journal of Clinical Microbiology 2007;45(4):1366-1369.
    22 Almenara Michelena C, del Buey Má, Ascaso FJ, Cristóbal Já. Keratitis due to achromobacter xylosoxidans in a contact lens user. Eye & Contact Lens: Science & Clinical Practice 2017:1.
    23 Loperena L, Soria V, Varela H, Lupo S, Bergalli A, Mairan GG, Pellegrino A, Bernardo A, Calviño A, Rivas F, Batista S. Extracellular enzymes produced by microorganisms isolated from maritime Antarctica. World J Microbiol Biotechnol 2012;28(5):2249-2256.
    24 Bibi F, Ullah I, Alvi SA, Bakhsh SA, Yasir M, Al-Ghamdi AAK, Azhar EI. Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia. Genet Mol Res 2017;16(2).
    25 Lowrey L, Woodhams DC, Tacchi L, Salinas I. Topographical mapping of the rainbow trout (oncorhynchus mykiss) microbiome reveals a diverse bacterial community with antifungal properties in the skin. Appl Environ Microbiol 2015;81(19):6915-6925.
    26 Findley K, Oh J, Yang J, Conlan S, Deming C, Meyer JA, Schoenfeld D, Nomicos E, Park M, Kong HH, Segre JA. Topographic diversity of fungal and bacterial communities in human skin. Nature 2013;498(7454):367-370.
    27 Iliev ID, Funari VA, Taylor KD, et al. Interactions between commensal fungi and the C-type lectin receptor Dectin-1 influence colitis. Science 2012;336(6086):1314-1317.
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Cheng Ge, Chao Wei, Bao-Xia Yang,/et al.Conjunctival microbiome changes associated with fungal keratitis: metagenomic analysis. Int J Ophthalmol, 2019,12(2):194-200

Copy
Share
Article Metrics
Article has an altmetric score of 9
Article has an altmetric score of 9
  • Abstract:1503
  • PDF: 851
  • HTML: 393
  • Cited by: 0
Publication History
  • Received:September 12,2018
  • Revised:November 07,2018
  • Online: January 03,2019
You are the 4480319 Visitors
Governing Body：Department of Health, Provincial Government of Shaanxi, China
Organizer：Xi'an Medical Association of China
Address：Room 11707-08, Building 2, Unit 1, Wanda Plaza, No.8 Yanta North Road, Xi'an 710054, Shaanxi Province, China
Fax：(8629)82245172 Phone：(+8629)82245172/82210956
Email：ijopress@163.com
International Journal of Ophthalmology ® 2025 All Rights Reserved   The terms of CC BY-NC-ND 4.0 are applicable to all open access content.