Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis
Author:
Corresponding Author:

Gui-Qiu Zhao. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao 266003, Shandong Province, China. zhaoguiqiu_good@126.com

Affiliation:

Clc Number:

Fund Project:

Supported by the National Natural Science Foundation of China (No.81470609; No.81700800; No.81870632; No.81800800); the Youth National Natural Science Foundation of China (No.81500695); Natural Science Foundation of Shandong Province (No.ZR2013HQ007; No.ZR2017MH008; No.ZR2017BH025).

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To investigate the expression and role of calcitonin gene-related peptide (CGRP) in the mouse models induced by Aspergillus fumigatus (A. fumigatus). METHODS: C57BL/6 mice were randomized into a control group and A. fumigatus keratitis group. The cornea photography was assessed under the slit lamp and the clinical score was recorded after infection. Western blot, real-time polymerase chain reaction (PCR) and immunohistofluorescence analysis were applied to detect CGRP expression in cornea of both groups. In vitro, tests were conducted with C57BL/6 mice macrophages to investigate CGRP expression after interaction with A. fumigatus. Cytokines expression induced by exogenous CGRP and the antagonist CGRP8-37 in A. fumigatus-exposed macrophages was evaluated by real-time PCR and ELISA. RESULTS: The cornea expression of CGRP was significantly elevated in C57BL/6 mice corneas and macrophages after A. fumigatus infection. After treatment with exogenous CGRP, the levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-6 were reduced, and IL-10 level was increased in the A. fumigatus stimulated-macrophages. However, IL-1β, TNF-α and IL-6 levels were upregulated after pretreatment of CGRP8-37. But the mRNA levels of MIP-2, TGF-β and IL-10 were not changed. CONCLUSION: This study provides evidence that A. fumigatus increased CGRP expression. CGRP may play a protective role against inflammation in A. fumigatus keratitis.

    Reference
    Related
    Cited by
Get Citation

Min Yin, Cui Li, Xu-Dong Peng, et al. Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis. Int J Ophthalmol, 2019,12(5):697-704

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:December 05,2018
  • Revised:March 08,2019
  • Adopted:
  • Online: March 27,2019
  • Published: