Effects of astaxanthin on antioxidant parameters in ARPE-19 cells on oxidative stress model
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Yi?it Musa. Department of Ophthalmology, Süleyman Demirel University Research and Education Hospital, ?ünür-Isparta 32200, Turkey. myigit15@gmail.com

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    Abstract:

    AIM: To observe the protective effect of astaxanthin (AST) against hydroquinone (HQ) mediated cell death in the apoptotic cascade and evaluate intracellular Ca2+ release, caspase-3, and -9 activation, reactive oxygen species (ROS) production in ARPE-19 cells. METHODS: We cultured ARPE-19 cells in special mediums and performed MTT tests to determine protective effect of AST, before exposing the cells to HQ in an incubator. We analyzed intracellular Ca2+ release experiments, mitochondrial membrane depolarization, glutathione (GSH), glutathione peroxidase (GSH-Px) and ROS experiments, and apoptosis assay. RESULTS: ROS production ranges depend on the amount of cell death. We computed the correlation between ROS ranges and cell death by 20,70-dichlorofluorescein fluorescence, and Ca2+ levels by Fura-2-AM. HQ-induced cell death found out to rise ranges of caspase-3 and -9, and mitochondrial depolarization. These three steps were delayed by AST management. CONCLUSION: ARPE-19 cells are avoided from HQ-induced ROS production and caspase-3 and -9 activation by AST. AST may limit the range of caspase synthesis, Ca2+ release and excess production of ROS with antiapoptotic effect. This study proposes a new therapeutic approach for the treatment of age-related macular degeneration.

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Yiğit M, Güneş A, Uğuz C, Yalçın TO, Tök L, Öz A, Nazıroğlu M. Effects of astaxanthin on antioxidant parameters in ARPE-19 cells on oxidative stress model. Int J Ophthalmol 2019;12(6):930-935

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Publication History
  • Received:April 07,2018
  • Revised:April 19,2019
  • Adopted:
  • Online: May 06,2019
  • Published: