High glucose: activating autophagy and affecting the biological behavior of human lens epithelial cells
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Pei-Rong Lu. Department of Ophthalmology, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China. lupeirong@suda.edu.cn

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Supported by the National Natural Science Foundation of China (No.81671641); the Natural Science Foundation of Jiangsu Province (No.BK20151208); Jiangsu Province’s Outstanding Medical Academic Leader Program (No.CXTDA2017039); Soochow Scholar Project of Soochow University (No.R5122001).

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    Abstract:

    AIM: To clarify the effect of autophagy on human lens epithelial cells (HLECs) under high glucose conditions. METHODS: HLECs were cultured with different concentrations of glucose and 3-methyladenine (3-MA); the expression of autophagy-related protein LC3B was detected by Western blotting and immunofluorescence histochemistry. The migration of HLECs was quantified by scratch wound assay and the expression of transforming growth factor-β (TGF-β) was measured by real-time polymerase chain reaction. RESULTS: Compared with 5 mmol/L normal glucose treatment, 40 mmol/L glucose treatment can significantly increase the generation of autophagosome in HLECs, which could be inhibited by 0.375 mmol/L 3-MA treatment. The migration of HLECs and the expression of TGF-β in HLECs induced by high glucose were significantly suppressed by 0.375 mmol/L 3-MA treatment. CONCLUSION: Autophagy promotes HLECs cell migration and increases the expression of TGF-β after exposed to high glucose, which may relate to the development of diabetic cataract.

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Dan Li, Gao-Qin Liu, Pei-Rong Lu. High glucose: activating autophagy and affecting the biological behavior of human lens epithelial cells. Int J Ophthalmol, 2019,12(7):1061-1066

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Publication History
  • Received:July 05,2018
  • Revised:October 22,2018
  • Adopted:
  • Online: June 11,2019
  • Published: